Vidigal T H, Spatz L, Nunes D N, Simpson A J, Carvalho O S, Dias Neto E
Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, Brazil.
Exp Parasitol. 1998 Jun;89(2):180-7. doi: 10.1006/expr.1998.4286.
The intermediate hosts of S. mansoni in South America, B. glabrata, B. tenagophila, and B. straminea, were identified by restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer region of the rRNA gene. The restriction patterns obtained with DdeI were the most informative of the eight enzymes that were tried. The RFLP profiles obtained using this enzyme are highly distinctive and exhibit low levels of intraspecific polymorphism even between specimens collected from diverse regions of Brazil, Argentine, Paraguay, and Uruguay. The method proved useful for the identification of DNA extracted from eggs, permitting species identification while preserving the living adult specimens for further studies.
南美洲曼氏血吸虫的中间宿主光滑双脐螺、嗜气管双脐螺和稻草双脐螺，通过对核糖体RNA基因内部转录间隔区的限制性片段长度多态性（RFLP）分析来鉴定。用DdeI获得的限制性图谱是所试验的8种酶中信息最多的。使用该酶获得的RFLP图谱具有高度特异性，即使在从巴西、阿根廷、巴拉圭和乌拉圭不同地区采集的标本之间，种内多态性水平也很低。该方法被证明对从虫卵中提取的DNA的鉴定很有用，在保留活体成虫标本以供进一步研究的同时能够进行物种鉴定。
