Tailoi C L, Gock B, Stone J
Department of Anatomy and Histology, University of Sydney, Australia.
Invest Ophthalmol Vis Sci. 1995 Jun;36(7):1215-30.
To determine the effectiveness of supplemental oxygen therapy (SOT) in ameliorating the proliferative vasculopathy characteristic of the feline model of retinopathy of prematurity (ROP).
Kittens were exposed to high (70% to 80%) oxygen for the first 4 days of life to induce obliteration of the retinal circulation. The level of inspired oxygen at successive stages after hyperoxia were varied systematically, and the retinas were examined for the extent of revascularization, astrocyte survival, intactness of the blood-retinal barrier, and extent of preretinal vessels.
The level of inspired oxygen required to protect the retina from hypoxic damage and yet provide a stimulus for growth of the vasculature varied during a 6-week period. The rate of revascularization of the retina increased as the oxygen content of the inspired gas mixture decreased, with a consequential increase in the pathology observed. However, a regimen that produced a quicker rate of revascularization with a slightly higher level of pathology actually offered the retina greater protection in the long term. The formation of preretinal vessels was effectively prevented by SOT. Supplemental oxygen therapy could be terminated once a significant portion of the retina was revascularized.
The proliferative vasculopathy of a kitten model of ROP can be significantly reduced by a regimen of SOT, which attempts to mimic physiological levels of hypoxia in the retina during its revascularization. Optimal revascularization requires a balance between the rate and quality of vessel growth. With further refinement of the protocol, SOT could provide noninvasive treatment of ROP in neonates.
确定补充氧气疗法（SOT）对改善早产儿视网膜病变（ROP）猫模型特征性增殖性血管病变的有效性。
小猫在出生后的头4天暴露于高浓度（70%至80%）氧气中，以诱导视网膜循环闭塞。高氧血症后连续阶段的吸入氧水平系统变化，检查视网膜的血管再形成程度、星形胶质细胞存活情况、血视网膜屏障完整性以及视网膜前血管程度。
在6周期间，保护视网膜免受缺氧损伤并为血管系统生长提供刺激所需的吸入氧水平有所变化。随着吸入气体混合物中氧含量降低，视网膜血管再形成速率增加，相应观察到的病理变化也增加。然而，一种血管再形成速率更快但病理水平略高的方案实际上从长期来看为视网膜提供了更大保护。SOT有效预防了视网膜前血管的形成。一旦视网膜的很大一部分实现血管再形成，补充氧气疗法即可终止。
ROP猫模型的增殖性血管病变可通过SOT方案显著降低，该方案试图在视网膜血管再形成期间模拟其生理缺氧水平。最佳的血管再形成需要在血管生长速率和质量之间取得平衡。随着方案的进一步完善，SOT可为新生儿ROP提供无创治疗。
