Rank R G, Dascher C, Bowlin A K, Bavoil P M
Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Invest Ophthalmol Vis Sci. 1995 Jun;36(7):1344-51.
To determine whether immunization with recombinant Hsp60 would exacerbate ocular pathology on challenge with viable chlamydial elementary bodies.
Guinea pigs were immunized either subcutaneously with recombinant Hsp60 or both subcutaneously with recombinant Hsp60 and ocularly with attenuated Salmonella typhimurium expressing the guinea pig inclusion conjunctivitis (GPIC) Hsp60 antigen. All animals were challenged in the conjunctiva with the agent of GPIC, and the degree of gross ocular pathology was determined. Immunoglobulin G (IgG) and immunoglobulin A (IgA) antibody titers to Hsp60 were measured in ocular secretions as a measure of the degree of immunization.
In primary and challenge GPIC infection, the degree of gross ocular pathology was lower in the immunized group. The presence of high IgA and IgG antibody titers to Hsp60 in tears suggested that the response may have been modified by the presence of blocking antibodies that either may have removed the antigen quickly or prevented interaction with sensitized T cells. In contrast to subcutaneous immunization, the combined immunization regimen, consisting of subcutaneous recombinant Hsp60 followed by ocular inoculation of the attenuated Salmonella, resulted in no difference in gross pathology after reinfection of guinea pigs with GPIC.
These data indicated that the immunization with Hsp60 did not produce exacerbated disease on challenge with viable organisms; however, the data suggested that the route of administration, form of antigen, or both may be critical in the disease process.
确定用重组热休克蛋白60（Hsp60）免疫是否会在受到活的衣原体原体攻击时加重眼部病变。
豚鼠分别皮下注射重组Hsp60，或同时皮下注射重组Hsp60并眼内接种表达豚鼠包涵体结膜炎（GPIC）Hsp60抗原的减毒鼠伤寒沙门氏菌。所有动物均用GPIC病原体攻击结膜，并确定眼部大体病变程度。检测眼部分泌物中针对Hsp60的免疫球蛋白G（IgG）和免疫球蛋白A（IgA）抗体滴度，作为免疫程度的指标。
在原发性和激发性GPIC感染中，免疫组的眼部大体病变程度较低。泪液中存在高滴度的针对Hsp60的IgA和IgG抗体，提示该反应可能因存在阻断抗体而改变，这些阻断抗体可能迅速清除了抗原或阻止了与致敏T细胞的相互作用。与皮下免疫不同，由皮下注射重组Hsp60随后眼内接种减毒沙门氏菌组成的联合免疫方案，在豚鼠再次感染GPIC后，大体病变无差异。
这些数据表明，用Hsp60免疫在受到活生物体攻击时不会产生加重的疾病；然而，数据表明给药途径、抗原形式或两者在疾病过程中可能至关重要。
