von Unge M, Decraemer W F, Dirckx J J, Bagger-Sjöbäck D
Department of Otorhinolaryngology, Karolinska Sjukhuset, Stockholm, Sweden.
Hear Res. 1995 Feb;82(2):184-96. doi: 10.1016/0378-5955(94)00017-k.
This study assesses the visco-elastic properties of the tympanic membrane (TM) in isolated gerbilline temporal bones as a function of time after inducing experimental otitis media with effusion (OME). To do this we measured the TM displacements produced by application of sequences of static pressures across the TM, with a high resolution, real-time, differential moiré interferometer, and the results were compared with measurements on healthy ears. Two methods of producing OME were used: in one group tubal plugging was performed to produce mild OME (the 'TP group'); in the other group electro-cauterization of the nasopharyngeal orifice of the Eustachian tube was used to cause a severe form of OME (the 'EC group'). The measurements were performed from one day up to ten weeks after surgery. In the TP group the displacement fringe patterns were normal, i.e. qualitatively they resembled the patterns of the control group. Quantitatively there was a significant decrease of displacement for a given pressure on the first day after surgery, followed by a trend of increase with time; after seven to ten days the displacement was larger than in the control group. In the EC group the displacement was significantly reduced after half a week, followed by a trend of increase with time, similar to what was found in the TP group; at one week the displacement was larger than in the control group, and at ten weeks the largest displacement was recorded. In the EC group the displacement patterns were often irregular; in some cases with changes suggesting the presence of weak spots in the TM where retraction pockets most likely could develop. OME seems to affect the stiffness of the TM promptly so that it is a potential parameter for early diagnosis. The stiffness changes may, if measurable in the clinical situation, become prognostic parameters in the treatment of OME.
本研究评估了在诱发实验性中耳积液（OME）后，分离的沙鼠颞骨中鼓膜（TM）的粘弹性特性随时间的变化。为此，我们使用高分辨率、实时、差分莫尔干涉仪测量了跨鼓膜施加一系列静态压力所产生的鼓膜位移，并将结果与健康耳朵的测量结果进行比较。采用了两种产生OME的方法：一组进行咽鼓管堵塞以产生轻度OME（“TP组”）；另一组采用咽鼓管鼻咽口电灼术导致严重形式的OME（“EC组”）。测量在手术后一天至十周内进行。在TP组中，位移条纹图案正常，即定性上它们类似于对照组的图案。定量方面，术后第一天给定压力下的位移显著降低，随后随时间有增加趋势；七至十天后，位移大于对照组。在EC组中，位移在半周后显著降低，随后随时间有增加趋势，与TP组情况类似；一周时位移大于对照组，十周时记录到最大位移。在EC组中，位移图案通常不规则；在某些情况下，变化表明鼓膜中存在薄弱点，最有可能形成回缩袋。OME似乎会迅速影响鼓膜的硬度，因此它是早期诊断的一个潜在参数。如果在临床情况下可测量，硬度变化可能成为OME治疗中的预后参数。
