Ishikawa N
Department of Obstetrics and Gynecology, National Defense Medical College, Saitama.
Nihon Sanka Fujinka Gakkai Zasshi. 1995 May;47(5):457-64.
We examined the effect of experimental endometriosis (EE) on natural killer (NK) activities and fertilizability in the rat. EE was induced by autotransplantation of an endometrial fragment to the peritoneal wall. As a sham, abdominal fat tissue was implanted in the same way. NK activities of spleen cells were measured by means of a 4-hr 51Cr-release assay.
(1) NK activities were significantly lower in EE than those in the sham (p < 0.001). (2) NK activity was recovered by surgical excision of the endometrial implant, but not increased by the administration of Danazol (100 mg/kg/day) or Buserelin (25 micrograms/kg/day) for 4 weeks. (3) The supernatant of endometriotic tissue cultures markedly suppressed NK activity. (4) The number of pups with EE was significantly smaller than in the controls (p < 0.05), but no difference between the two groups was observed in the number of oocytes in the Fallopian tube.
(1) Splenic NK activity decreases at the time of the establishment of EE. (2) NK activity recovers after the removal of the implanted tissue. (3) Danazol and Buserelin have no effect on the NK activity in EE. (4) EE reveals some adverse effects on the postovulatory phase of fertility.
我们研究了实验性子宫内膜异位症（EE）对大鼠自然杀伤（NK）活性和受精能力的影响。通过将子宫内膜碎片自体移植到腹壁来诱导EE。作为假手术，以相同方式植入腹部脂肪组织。通过4小时51Cr释放试验测量脾细胞的NK活性。
（1）EE组的NK活性显著低于假手术组（p < 0.001）。（2）通过手术切除子宫内膜植入物可使NK活性恢复，但连续4周给予达那唑（100 mg/kg/天）或布舍瑞林（25 μg/kg/天）不会使其增加。（3）子宫内膜异位组织培养物的上清液显著抑制NK活性。（4）EE组的幼崽数量显著少于对照组（p < 0.05），但两组输卵管中的卵母细胞数量无差异。
（1）EE形成时脾NK活性降低。（2）去除植入组织后NK活性恢复。（3）达那唑和布舍瑞林对EE中的NK活性无影响。（4）EE对排卵后生育阶段有一些不利影响。
