Chen B F, Marrogi A J, Freeman S M, Clejan S
Tulane Medical Center, New Orleans, USA.
J La State Med Soc. 1995 Apr;147(4):138-45.
The prognosis of gastric carcinoma remains unfavorable despite a greater understanding of its molecular pathology. This retrospective study of primary gastric carcinomas was collected from one of the highest risk regions of China and examined for the oncogenetic expression of p53, c-erbB-2, and PCNA using immunohistochemistry and DNA contents by flow cytometry and image analysis. These products are reported to influence the tumor behavior. The p53 nuclear and c-erbB-2 membrane-bound stainings were seen in 58% and 34% of cases, respectively. A high PCNA index was found in 90% of the tumors. The p53 expression did not correlate with the histological differentiation, gross morphology, and depth of tumor invasion. Additionally, p53 and c-erbB-2 reactivity did not correlate with the proliferative index (PI) or S-phase DNA content. However, the mutant p53 expression was detected in the dysplastic cells adjacent to the tumor, suggesting a possible role of the oncogene in tumor pathogenesis. Mutant p53 expression can also be helpful in early detection of cases with dysplasia in well-differentiated adenocarcinomas.
尽管对胃癌的分子病理学有了更深入的了解，但其预后仍然不容乐观。这项对原发性胃癌的回顾性研究收集自中国胃癌高发地区之一，通过免疫组织化学检测了p53、c-erbB-2和PCNA的致癌基因表达，并采用流式细胞术和图像分析检测了DNA含量。据报道，这些产物会影响肿瘤行为。分别在58%和34%的病例中观察到p53核染色和c-erbB-2膜结合染色。在90%的肿瘤中发现了高PCNA指数。p53表达与组织学分化、大体形态和肿瘤浸润深度无关。此外，p53和c-erbB-2反应性与增殖指数（PI）或S期DNA含量无关。然而，在肿瘤邻近的发育异常细胞中检测到了突变型p53表达，提示该癌基因在肿瘤发病机制中可能发挥作用。突变型p53表达也有助于早期发现高分化腺癌中的发育异常病例。
