Clements G B, McGarry F, Nairn C, Galbraith D N
Regional Virus Laboratory, Ruchill Hospital, Glasgow, United Kingdom.
J Med Virol. 1995 Feb;45(2):156-61. doi: 10.1002/jmv.1890450208.
The serum of 88 chronic fatigue patients was screened for enteroviral specific sequences by polymerase chain reaction (PCR) assay. The PCR method used was "nested" PCR targetting the 5' nontranslated region of the enteroviral genome which yielded a final fragment length of 264 base pairs. Samples were obtained from patients during 1990-1991. In addition, buffy coat specimens and stool specimens were examined in some patients. Samples from two cohorts of comparison individuals were also obtained. The comparison groups were firstly, acutely ill individuals with symptoms consistent with a presumed enteroviral infection (matched by age, sex, and date of receipt of specimen) and secondly, healthy individuals (matched by age and date of receipt of specimen). Enteroviral specific sequences were detected in 36 of 88 serum samples from chronic fatigue patients, 22 of 82 acutely ill individuals, and 3 of 126 healthy individuals. The enteroviral PCR positivity did not correlate with any one particular feature of chronic fatigue nor did it reflect any history of illness at onset of fatigue, duration of fatigue, or age of patient. These results provide new evidence for the presence of enteroviral specific sequences in serum, buffy coat, and stool samples in many patients with chronic fatigue. This may reflect a persistent enterovirus infection in a proportion of chronic fatigue patients.
采用聚合酶链反应（PCR）检测法，对88例慢性疲劳患者的血清进行肠道病毒特异性序列筛查。所采用的PCR方法为“巢式”PCR，靶向肠道病毒基因组的5'非翻译区，最终扩增片段长度为264个碱基对。样本取自1990年至1991年期间的患者。此外，还对部分患者的血沉棕黄层标本和粪便标本进行了检测。同时获取了两组对照个体的样本。第一组对照组为有疑似肠道病毒感染症状的急性病患者（按年龄、性别和标本采集日期匹配），第二组为健康个体（按年龄和标本采集日期匹配）。在88例慢性疲劳患者的血清样本中，有36例检测到肠道病毒特异性序列；82例急性病患者中有22例；126例健康个体中有3例。肠道病毒PCR阳性结果与慢性疲劳的任何一项特定特征均无关联，也未反映出疲劳发作时的任何病史、疲劳持续时间或患者年龄。这些结果为许多慢性疲劳患者的血清、血沉棕黄层和粪便样本中存在肠道病毒特异性序列提供了新证据。这可能反映出一部分慢性疲劳患者存在持续性肠道病毒感染。
