Boretti G, Zappa U, Graf H, Case D
Department of Prosthodontics, Dental School, University of Bern, Switzerland.
J Periodontol. 1995 Mar;66(3):235-40. doi: 10.1902/jop.1995.66.3.235.
The purpose of the present study was to use a novel intracrevicular lavage technique to evaluate short-term effects of phase I therapy on crevicular cell populations. Nineteen patients with untreated advanced adult periodontitis were selected for phase I therapy. One side of the dentition was treated with ultrasonic curets (U), the other side with manual curets (M). Nine months before (-9), immediately prior to (0), and 1 month after treatment (+1) gingival index (GI), plaque index (PI), bleeding index (BI), bleeding on probing (BOP), probing depth (PD), and probing attachment levels (PAL) were measured at all sites in the dentition. Crevicular lavages were obtained from 3 to 4 selected sites per patient at the same time points. Crevicular leukocytes were vital stained with ethydium bromide-fluorescein-diacetate (EB-FDA). The total number of cells and the percentage of vital cells (%) were calculated for each sample. Clinical and lavage parameters obtained from the selected sites were compared between U and M sites, and between pre- and post-treatment values. The results showed that without treatment PAL remained at the same level at both pretreatment time points -9 and -0 (control). At 1 month after treatment there were statistically significant reductions in GI, PI, BOP, and PD (P < 0.001 for each comparison), and a statistically significant gain in PAL from 4.9 to 4.1 mm (P = 0.014). The total number of leukocytes per sample was similar at both pretreatment time points, but numerically reduced at 1 month after treatment. The percent of vital leukocytes was above 74% at both pretreatment time points (control). After treatment these values were below 70%. This reduction was statistically significant (P < 0.002). These results suggest that periodontal phase I therapy leads to shifts in crevicular cell populations.
本研究的目的是使用一种新型的龈沟内灌洗技术来评估I期治疗对龈沟细胞群体的短期影响。选取19例未经治疗的重度成人牙周炎患者进行I期治疗。牙列的一侧用超声刮治器（U）治疗，另一侧用手动刮治器（M）治疗。在治疗前9个月（-9）、治疗前即刻（0）和治疗后1个月（+1），测量牙列所有部位的牙龈指数（GI）、菌斑指数（PI）、出血指数（BI）、探诊出血（BOP）、探诊深度（PD）和探诊附着水平（PAL）。在相同时间点，从每位患者的3至4个选定部位获取龈沟灌洗液。龈沟白细胞用溴化乙锭 - 荧光素二乙酸酯（EB - FDA）进行活细胞染色。计算每个样本的细胞总数和活细胞百分比（%）。比较U组和M组部位以及治疗前后值之间从选定部位获得的临床和灌洗参数。结果显示，在两个预处理时间点 -9和 -0（对照）时，未经治疗的PAL保持在相同水平。治疗后1个月，GI、PI、BOP和PD有统计学显著降低（每次比较P < 0.001），PAL从4.9毫米有统计学显著增加至4.1毫米（P = 0.014）。每个样本的白细胞总数在两个预处理时间点相似，但在治疗后1个月在数值上减少。两个预处理时间点（对照）的活白细胞百分比均高于74%。治疗后这些值低于70%。这种降低具有统计学显著性（P < 0.002）。这些结果表明牙周I期治疗导致龈沟细胞群体发生变化。
