Wang K Y, Chen K C, Chiang C P, Kuo M Y
School of Dentistry, National Taiwan University, Taipei, ROC.
J Oral Pathol Med. 1995 Mar;24(3):103-8. doi: 10.1111/j.1600-0714.1995.tb01148.x.
Cleft lip, with or without cleft palate, is one of the most common defects in craniofacial formation. The primary palatogenesis of mice is similar to that of humans and spontaneous cleft lip is associated with genotype in both mice and humans. To investigate the temporal and spatial expression of ras genes in cleft (A/WySn) and non-cleft strains of mice (BALB/cBy), a broad spectrum ras antibody was used. Positive staining was found in ectodermal, mesenchymal, and neuroepithelial cells of facial prominences before the primary palate formation stage (10 d 20 hr) in both strains. During the primary palate formation stage (11 d 20 hr), positive staining was found in the ectodermal and mesenchymal cells of the facial prominences of the non-cleft strain but not in those of the cleft strain. These results suggest ras genes may play a role in the primary palatogenesis of mice. Cleft lip could be associated with the deficiency of ras gene expression during primary palate formation of mice.
唇裂，伴或不伴腭裂，是颅面形成中最常见的缺陷之一。小鼠的原发性腭发育与人类相似，并且在小鼠和人类中，自发性唇裂均与基因型有关。为了研究ras基因在腭裂（A/WySn）和非腭裂小鼠品系（BALB/cBy）中的时空表达，使用了一种广谱ras抗体。在两个品系中，在原发性腭形成阶段（10天20小时）之前，在面部隆起的外胚层、间充质和神经上皮细胞中发现了阳性染色。在原发性腭形成阶段（11天20小时），在非腭裂品系的面部隆起的外胚层和间充质细胞中发现了阳性染色，但在腭裂品系中未发现。这些结果表明ras基因可能在小鼠的原发性腭发育中起作用。唇裂可能与小鼠原发性腭形成过程中ras基因表达的缺乏有关。
