Yamaguchi K, Ozono Y, Harada T, Hara K
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Nephron. 1995;69(4):384-90. doi: 10.1159/000188507.
The circulating IgA class immune complex (IgA-IC) and its charge distribution, at the appearance of macroscopic hematuria and after tonsillectomy in patients with IgA nephropathy, were investigated in the present study. 3.5% polyethylene glycol precipitate (3.5% PEG-IgA) was used for IgA-IC detection and isoelectric focusing for its charge distribution. The level of IgA in 3.5% PEG-IgA, principally IgA1, and the proportion of anionic 3.5% PEG-IgA (isoelectric point, pl, 4.8-5.6) were significantly elevated in episodes of macroscopic hematuria with upper respiratory tract inflammation and with the appearance of macroscopic hematuria 1 day after tonsillectomy. Therefore, an increase in anionic IgA-IC (pl 4.8-5.6), principally IgA1, and the tonsils were considered to be concerned with the mechanism involved in the appearance of macroscopic hematuria.
本研究调查了IgA肾病患者出现肉眼血尿时以及扁桃体切除术后循环IgA类免疫复合物（IgA-IC）及其电荷分布情况。采用3.5%聚乙二醇沉淀法（3.5% PEG-IgA）检测IgA-IC，并通过等电聚焦分析其电荷分布。在上呼吸道炎症伴肉眼血尿发作时以及扁桃体切除术后1天出现肉眼血尿时，3.5% PEG-IgA中的IgA水平（主要为IgA1）以及阴离子3.5% PEG-IgA（等电点，pl，4.8 - 5.6）的比例显著升高。因此，阴离子IgA-IC（pl 4.8 - 5.6），主要是IgA1的增加以及扁桃体被认为与肉眼血尿出现的机制有关。
