Yew D T, Luo C B, Shen W Z, Chow P H, Zheng D R, Yu M C
Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T.
Neuroscience. 1995 Mar;65(2):453-61. doi: 10.1016/0306-4522(94)00521-6.
Six human fetuses of gestational ages 16-28 weeks were employed. The immunocytochemical avidin-biotin-peroxidase complex method combined with the silver Bodian technique was used to evaluate the presence of tyrosine hydroxylase and dopamine-beta-hydroxylase neurons and afferent and efferent fibres in the cerebellum during development. Our results illustrated that by 16-18 weeks, immunoreactivity of the Purkinje cells and the granule cells was evident. By 23 weeks, the positive Purkinje cells were tightly packed together and the perinuclear granules began to extend into the processes. The positive cells next to Purkinje cells were the basket cells and stellate cells. By 26-28 weeks, all positive cells increased in number and size. Mossy and climbing fibres appeared early in development (16-18 weeks of gestation) and were seen synapsing with the positive granule cells. At the same time, some parallel fibres were observed. At later stages, the tyrosine hydroxylase- and dopamine-beta-hydroxylase-positive Purkinje cells were surrounded by abundant climbing fibres, while parallel fibres were also evident in the molecular layer. In the deep cerebellar nuclei, positive tyrosine hydroxylase and dopamine-beta-hydroxylase neurons were present by 16-18 weeks of development. Those in the dentate nucleus were more polymorphic but smaller in size. Some afferent fibres were also spotted around 16-18 weeks of gestation and their numbers increased later. Positive efferent fibres were present by 26 weeks. All these observations point to an early presence of tyrosine hydroxylase and dopamine-beta-hydroxylase components in cerebellar development.
使用了6例孕龄为16 - 28周的人类胎儿。采用免疫细胞化学抗生物素蛋白-生物素-过氧化物酶复合物法结合银染博迪安技术，来评估小脑在发育过程中酪氨酸羟化酶和多巴胺-β-羟化酶神经元以及传入和传出纤维的存在情况。我们的结果表明，在16 - 18周时，浦肯野细胞和颗粒细胞的免疫反应性明显。到23周时，阳性浦肯野细胞紧密聚集在一起，核周颗粒开始延伸至突起。浦肯野细胞旁边的阳性细胞是篮状细胞和星形细胞。到26 - 28周时，所有阳性细胞的数量和大小都增加了。苔藓纤维和攀缘纤维在发育早期（妊娠16 - 18周）出现，并可见与阳性颗粒细胞形成突触。同时，观察到一些平行纤维。在后期，酪氨酸羟化酶和多巴胺-β-羟化酶阳性的浦肯野细胞被丰富的攀缘纤维包围，而分子层中平行纤维也很明显。在小脑深部核团中，在发育16 - 18周时就存在阳性的酪氨酸羟化酶和多巴胺-β-羟化酶神经元。齿状核中的神经元形态更多样，但体积较小。在妊娠16 - 18周左右也发现了一些传入纤维，其数量随后增加。在26周时出现了阳性传出纤维。所有这些观察结果表明，酪氨酸羟化酶和多巴胺-β-羟化酶成分在小脑发育中早期就存在。
