Tillet Y, Thibault J
Unité de Neuroendocrinologie Sexuelle, INRA Station de Physiologie de la Reproduction, Nouzilly, France.
J Chem Neuroanat. 1993 Mar-Apr;6(2):69-78. doi: 10.1016/0891-0618(93)90029-4.
Double immunocytochemical labelling with antibodies raised against tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase was used on semi-thin sections of sheep hypothalamus to investigate possible morphological relationships between dopamine neurons of group A15 and noradrenaline afferents to this area. Dopamine-beta-hydroxylase-immunoreactive (IR) fibres were found in the close proximity of dendrites of TH-IR neurons. At electron microscopic level, single immunocytochemical staining with TH antibodies revealed the presence of synaptic contacts between labelled or unlabelled axon terminals and anti-TH labelled dendrites. These observations suggest that in the sheep, TH-IR neurons of group A15 are controlled by non-catecholaminergic and catecholaminergic afferents. Catecholamine inputs could contain either dopamine or noradrenaline. The hypothesis of noradrenaline inputs to A15 is strongly supported by the results obtained after double labelling on semi-thin sections. Tyrosine hydroxylase-immunoreactive perikarya and dendrites often seemed to be partly surrounded by glial processes. This latter observation suggests that the synaptic investment of these neurons might be controlled by glial cells.
使用针对酪氨酸羟化酶（TH）和多巴胺-β-羟化酶产生的抗体，对绵羊下丘脑的半薄切片进行双重免疫细胞化学标记，以研究A15组多巴胺能神经元与该区域去甲肾上腺素能传入神经之间可能的形态学关系。在TH免疫反应性（IR）神经元的树突附近发现了多巴胺-β-羟化酶免疫反应性（IR）纤维。在电子显微镜水平上，用TH抗体进行的单免疫细胞化学染色显示，标记或未标记的轴突终末与抗TH标记的树突之间存在突触联系。这些观察结果表明，在绵羊中，A15组的TH-IR神经元受非儿茶酚胺能和儿茶酚胺能传入神经的控制。儿茶酚胺输入可能包含多巴胺或去甲肾上腺素。半薄切片双重标记后获得的结果有力支持了去甲肾上腺素输入到A15的假说。酪氨酸羟化酶免疫反应性胞体和树突似乎常常部分被神经胶质细胞突起包围。后一观察结果表明，这些神经元的突触包裹可能受神经胶质细胞控制。
