Naito M, Umegaki H, Iguchi A
Department of Geriatrics, Nagoya University School of Medicine, Japan.
Neurosci Lett. 1995 Feb 17;186(2-3):211-3. doi: 10.1016/0304-3940(95)11321-m.
The sympathetic nerve cell line PC12 is reportedly destroyed by glutamate in a concentration-dependent manner. In this study, cytotoxicity induced by 10 mM glutamate was blocked in a dose-dependent manner by incubating or pre-incubating cells with the antioxidant probucol (1-50 microM). Probucol also inhibited the increase in cellular thiobarbituric acid-reacting substances induced by glutamate exposure. These results suggest that the propagation of cellular lipid peroxidation may be related to glutamate-induced toxicity in PC12 cells.
据报道，交感神经细胞系PC12会被谷氨酸以浓度依赖的方式破坏。在本研究中，通过用抗氧化剂普罗布考（1-50微摩尔）孵育或预孵育细胞，10毫摩尔谷氨酸诱导的细胞毒性以剂量依赖的方式被阻断。普罗布考还抑制了谷氨酸暴露诱导的细胞硫代巴比妥酸反应物质的增加。这些结果表明，细胞脂质过氧化的传播可能与PC12细胞中谷氨酸诱导的毒性有关。
