Mitalipov Sh M, Mitallipova M M, Ivanov V I
Ontogenez. 1994 Nov-Dec;25(6):19-27.
A comparative analysis of two clones of mouse embryonic stem cells (ES-D3) that underwent different number of passages was performed to determine their potencies for in vitro and in vivo development. Cells of both clones had similar morphology characteristic of undifferentiated ES cells and were capable of forming embryoid bodies in the suspension cultures. Specific alkaline phosphatase activity of ES cells was revealed by cytochemical staining. Karyotyping showed that the proportion of aneuploid ES cells increases with an increase in the number of passages. The results of experiments on chimera production using ES cells showed that the clone D3W (passage 17) is superior to the clone D3M (passage 42) in terms of both the proportion of chimeras produced and the degree of coat color chimerism in them.
对经历不同传代次数的两个小鼠胚胎干细胞克隆（ES-D3）进行了比较分析，以确定它们在体外和体内发育的潜能。两个克隆的细胞都具有未分化ES细胞相似的形态特征，并且能够在悬浮培养中形成胚状体。通过细胞化学染色显示了ES细胞的特异性碱性磷酸酶活性。核型分析表明，非整倍体ES细胞的比例随着传代次数的增加而增加。使用ES细胞进行嵌合体生产的实验结果表明，就产生的嵌合体比例及其毛色嵌合程度而言，克隆D3W（第17代）优于克隆D3M（第42代）。
