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Simple and efficient method for isolation and measurement of cyanobacterial hepatotoxins by plant tests (Sinapis alba L.).

作者信息

Kós P, Gorzó G, Surányi G, Borbély G

机构信息

Institute of Plant Biology, Biological Research Center, Szeged, Hungary.

出版信息

Anal Biochem. 1995 Feb 10;225(1):49-53. doi: 10.1006/abio.1995.1106.

DOI:10.1006/abio.1995.1106
PMID:7778786
Abstract

A simple and cost-effective method for isolating and assaying microcystins, cyanobacterial toxins, by C-18 cartridges, DEAE-cellulose (DE-52) chromatography, and a mustard (Sinapis alba L.) plant seedling test is described. The procedure results in a purity of up to 95-97% microcystin without the need for an HPLC system and justifies the use of the S. alba L. seedling test in the quantitative assessment of the toxin with an IC50 of 3 micrograms ml-1 instead of the mouse intraperitoneal test.

摘要

相似文献

1
Simple and efficient method for isolation and measurement of cyanobacterial hepatotoxins by plant tests (Sinapis alba L.).
Anal Biochem. 1995 Feb 10;225(1):49-53. doi: 10.1006/abio.1995.1106.
2
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