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微囊藻毒素的摄取抑制芥菜(白芥)幼苗的生长和蛋白磷酸酶活性。

Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings.

作者信息

Kurki-Helasmo K, Meriluoto J

机构信息

Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.

出版信息

Toxicon. 1998 Dec;36(12):1921-6. doi: 10.1016/s0041-0101(98)00114-7.

DOI:10.1016/s0041-0101(98)00114-7
PMID:9839676
Abstract

Mustard (Sinapis alba L.) seeds were cultivated for seven days on a solid nutrient medium supplemented with 040 microg microcystin-RR per ml. Microcystin-RR affected seedling growth (IC50 0.8 microg/ml) and microcystin concentrations > or =5.0 microg/ml produced malformed plants. The inhibition of protein phosphatase 1 and 2A activity correlated with the growth inhibition. The seedlings were also shown to take up 3H-dihydromicrocystin-LR derived radioactivity up to a level corresponding to ca. 80 ng toxin per mg plant protein.

摘要

芥菜(白芥)种子在每毫升添加了040微克微囊藻毒素-RR的固体营养培养基上培养7天。微囊藻毒素-RR影响幼苗生长(半数抑制浓度为0.8微克/毫升),微囊藻毒素浓度≥5.0微克/毫升时会产生畸形植株。蛋白磷酸酶1和2A活性的抑制与生长抑制相关。还发现幼苗能够吸收3H-二氢微囊藻毒素-LR衍生的放射性物质,其水平相当于每毫克植物蛋白约80纳克毒素。

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