Rogalla P, Rohen C, Hennig Y, Deichert U, Bonk U, Bullerdiek J
Centre of Human Genetics and Genetic Counselling, University of Bremen, Germany.
Biochem Biophys Res Commun. 1995 Jun 6;211(1):175-82. doi: 10.1006/bbrc.1995.1793.
We have compared the length of telomere repeat fragments (TRF's) in 19 uterine leiomyomas from 6 patients with the corresponding myometrium. The advantage of this study of TRF length is that cells from uterine leiymyoma and cells from corresponding myometrium do not contain any considerable proportions of other cells as revealed by analysis of clonality. In all tumor samples a loss of TRF length ranging from 1120 to 4690 bp was noted. There was no correlation between tumor volume or size of tumor population as revealed by histological examination and loss of TRF length. From the obtained TRF length data (an average myometrial TFR length of 13 kb and an average loss of TRF length in myoma cells of 83 bp per cell division) we concluded that TRF length reduction does not limit the growth potential of uterine leiomyomas.
我们比较了6例患者的19个子宫平滑肌瘤及其相应子宫肌层中端粒重复片段（TRF）的长度。这项关于TRF长度的研究的优势在于，通过克隆性分析发现，子宫平滑肌瘤细胞和相应子宫肌层细胞中不含有任何占相当比例的其他细胞。在所有肿瘤样本中，均观察到TRF长度减少了1120至4690 bp。组织学检查显示，肿瘤体积或肿瘤细胞群大小与TRF长度减少之间无相关性。根据获得的TRF长度数据（子宫肌层TRF平均长度为13 kb，肌瘤细胞中TRF长度平均每细胞分裂减少83 bp），我们得出结论，TRF长度减少并不限制子宫平滑肌瘤的生长潜能。
