Zhong Ying-Cheng, Luo Peng-Li, Jin Guo-En, Yang Hui-Lian, Han Shu-Fen, Ge Ri-Li
High Altitude Medical Research Center, Qinghai University Medical College, Xining 810001, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2008 Jul;42(7):502-5.
To investigate the relationship between the length of telomere DNA and age at different altitude areas.
All 172 peripheral blood samples were randomly selected from healthy individuals of different ages from 25 to 65 years old. High altitude group (47 males, 48 females) living at an altitude of 4380 m (HA group), sea level group (39 males, 38 females) living at an altitude of 43 m (SL group). The terminal restriction fragment (TRF) length of telomere DNA was measured by Southern blotting analysis. The plasma levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were assayed.
Average TRF lengths of males and females in HA groups were 10.45 +/- 1.35 and 10.50 +/- 1.45. Average TRF lengths of males and females in SL groups were 11.29 +/- 1.10 and 11.31 +/- 1.13. A negative correlation was shown between the average TRF length and age of males in two groups (P < 0.01). This was also the case for females. ANOVA test was used to analyze the difference between TRF length and gender at different ages (P < 0.001). It was shown that there was significant difference in TRF length between the male (25 years old and 55 years old) and female (25 years old and 55 years old) in two groups at different ages (P < 0.05). The plasma levels of SOD and MDA were significant different between HA groups and SL groups (25-44 years old groups/45-65 years old groups) (P < 0.05).
Obviously shortening of telomere was observed by increasing of ages in high altitude groups. There was a negative correlation between the length of telomere DNA and ages. Telomere shortening became more obviously in high altitude group than in sea level group in keeping with the age increases.
探讨不同海拔地区端粒DNA长度与年龄之间的关系。
从25至65岁的不同年龄段健康个体中随机选取172份外周血样本。高海拔组（47例男性，48例女性）生活在海拔4380米处（HA组），海平面组（39例男性，38例女性）生活在海拔43米处（SL组）。通过Southern印迹分析测量端粒DNA的末端限制片段（TRF）长度。检测血浆丙二醛（MDA）和超氧化物歧化酶（SOD）水平。
HA组男性和女性的平均TRF长度分别为10.45±1.35和10.50±1.45。SL组男性和女性的平均TRF长度分别为11.29±1.10和11.31±1.13。两组男性的平均TRF长度与年龄呈负相关（P<0.01）。女性也是如此。采用方差分析检验不同年龄组TRF长度与性别的差异（P<0.001）。结果显示，两组不同年龄的男性（25岁和55岁）和女性（25岁和55岁）之间的TRF长度存在显著差异（P<0.05）。HA组和SL组（25 - 44岁组/45 - 65岁组）的血浆SOD和MDA水平存在显著差异（P<0.05）。
高海拔组中随着年龄增长端粒明显缩短。端粒DNA长度与年龄呈负相关。随着年龄增长，高海拔组端粒缩短比海平面组更明显。
