Padbury J F, Tseng Y T, Waschek J A
Department of Pediatrics, Harbor-UCLA Medical Center, USA.
Biochem Biophys Res Commun. 1995 Jun 6;211(1):254-61. doi: 10.1006/bbrc.1995.1804.
We cloned and sequenced the ovine beta 1 adrenergic receptor (beta 1AR) gene and promoter region. The transcription start site was localized by RNase protection and primer extension to a GC-rich region. The predominant initiation sequence did not resemble an initiator element and there was no upstream TATA box. Sequence analysis revealed several potential thyroid hormone and glucocorticoid regulatory elements. Identification of the promoter structure of this important gene will help define its novel regulation during development.
我们克隆并测序了绵羊β1肾上腺素能受体（β1AR）基因及其启动子区域。通过核糖核酸酶保护法和引物延伸法将转录起始位点定位到一个富含GC的区域。主要的起始序列与起始子元件并不相似，且上游没有TATA框。序列分析揭示了几个潜在的甲状腺激素和糖皮质激素调节元件。鉴定这个重要基因的启动子结构将有助于明确其在发育过程中的新调控机制。
