Lee Y J, Chung T J, Park C W, Hahn Y, Chung J H, Lee B L, Han D M, Jung Y H, Kim S, Lee Y
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, Korea.
Biochem Biophys Res Commun. 1996 Jan 5;218(1):6-11. doi: 10.1006/bbrc.1996.0002.
A genomic DNA fragment encoding tenecin 3, an antifungal protein was cloned from the genomic DNA library of Tenebrio molitor. The DNA sequence analysis showed that the coding region is divided into two exons by an intron of 49 bp in the middle of the putative leader peptide coding region. Southern blot analysis suggests that the gene is present as a single copy. The transcription initiation site was determined by primer extension analysis and S1 mapping. The TATA box and CCAAT box sequences were found at -30, and -121, respectively, from the transcription initiation site. Tenecin 3 mRNA is abundantly expressed in larvae and adults, while little was detected in RNAs from pupae, suggesting that the expression of the tenecin 3 gene is developmentally regulated.
从黄粉虫的基因组DNA文库中克隆出一个编码抗真菌蛋白天蚕素3的基因组DNA片段。DNA序列分析表明，编码区在假定的前导肽编码区中部被一个49bp的内含子分为两个外显子。Southern印迹分析表明该基因以单拷贝形式存在。通过引物延伸分析和S1图谱确定了转录起始位点。在距转录起始位点分别为-30和-121处发现了TATA盒和CCAAT盒序列。天蚕素3 mRNA在幼虫和成虫中大量表达，而在蛹的RNA中检测到的很少，这表明天蚕素3基因的表达受发育调控。
