Mateescu M A, Chahine R, Roger S, Atanasiu R, Yamaguchi N, Lalumière G, Nadeau R
Department of Chemistry-Biochemistry, Université du Québec á Montréal, Canada.
Arzneimittelforschung. 1995 Apr;45(4):476-80.
This study describes the carioprotective effect of ceruloplasmin (CAS 9031-37-2) against oxygen free radical injury, as indicated by several biochemical indicators and some cardiodynamic variables. Isolated rat hearts (n = 4-8, p < 0.05, for each experimental point) in Langendorff preparation were exposed to oxygen free radicals generated by electrolysis (10 mA) in the absence and the presence of 0.25 mumol/l purified ceruloplasmin and denaturated ceruloplasmin, in Krebs-Henseleit perfusion solutions. Biochemical indicators (noradrenaline, malondialdehyde, creatine-kinase, lactate dehydrogenase, aspartate aminotransferase, Ca2+ and Mg2+) as well as the electrocardiogram and the left ventricular pressure (LVP), were altered by oxygen free radicals formation, denoting major cellular and tissular damages in the nontreated hearts. Ceruloplasmin exhibited a cardioprotective effect and prevented the oxygen free radical-induced release of noradrenaline, indicating that it can also protect the sympathetic nerve endings from oxygen free-radical injury. Purified ceruloplasmin, a circulating extracellular antioxidant and oxygen free radical scavenger, seems to be an effective heart protective agent against myocardial and neuronal injuries generated by oxygen free radicals.
本研究通过若干生化指标和一些心脏动力学变量，描述了铜蓝蛋白（CAS 9031-37-2）对氧自由基损伤的护齿作用。在Krebs-Henseleit灌注溶液中，将Langendorff制备的离体大鼠心脏（每个实验点n = 4-8，p < 0.05）在无和有0.25 μmol/l纯化铜蓝蛋白及变性铜蓝蛋白存在的情况下，暴露于电解（10 mA）产生的氧自由基中。氧自由基的形成改变了生化指标（去甲肾上腺素、丙二醛、肌酸激酶、乳酸脱氢酶、天冬氨酸转氨酶、Ca2+和Mg2+）以及心电图和左心室压力（LVP），表明未处理的心脏存在主要的细胞和组织损伤。铜蓝蛋白表现出心脏保护作用，并防止了氧自由基诱导的去甲肾上腺素释放，表明它还可以保护交感神经末梢免受氧自由基损伤。纯化的铜蓝蛋白是一种循环的细胞外抗氧化剂和氧自由基清除剂，似乎是一种有效的心脏保护剂，可抵御氧自由基产生的心肌和神经元损伤。
