Bertuglia S, Malandrino S, Colantuoni A
CNR-Consiglio Nazionale delle Ricerche, Institute of Clinical Physiology, Milan, Italy.
Arzneimittelforschung. 1995 Apr;45(4):481-5.
The purpose of the present study was to investigate the effects of the flavonoid delphinidin chloride (CAS 528-53-0, IdB 1056) on diabetic microangiopathy. Hamsters were injected with alloxan and cheek pouch microcirculation was observed by a fluorescent microscopy technique 90 days from alloxan. The increase in permeability, the number of adhering leukocytes to venular vessel wall and vasodilatory responses to acetylcholine (Ach) and sodium nitroprusside (SNP) were measured. In diabetic group microvascular permeability and the number of sticking leukocytes to the venular endothelium were increased. Vasoconstriction by Ach was observed while the vasodilation by SNP was significantly attenuated in diabetic animals. These results are consistent for a decreased relaxation and suggest also an impairment in the smooth muscle cell function in diabetic arterioles. IdB 1056 exhibited an inhibitory effect on increased microvascular permeability and on leukocytes adhering to the venular vessels. Indeed, the treatment with IdB 1056 in diabetic hamsters pretreated or not with indometacin, a cyclooxygenase inhibitor, restored the relaxant responses to Ach and SNP. In conclusion, the effects of IdB 1056 observed in vivo at the microcirculatory level prevent the injury to endothelial cell function associated with diabetes and/or oxidative stress.
本研究的目的是探讨氯化飞燕草素（CAS 528-53-0，IdB 1056）对糖尿病微血管病变的影响。给仓鼠注射四氧嘧啶，并在注射四氧嘧啶90天后通过荧光显微镜技术观察颊囊微循环。测量了微血管通透性的增加、贴壁白细胞数量以及对乙酰胆碱（Ach）和硝普钠（SNP）的血管舒张反应。在糖尿病组中，微血管通透性和贴壁白细胞数量增加。糖尿病动物中观察到Ach引起的血管收缩，而SNP引起的血管舒张则明显减弱。这些结果与舒张功能降低一致，也提示糖尿病小动脉平滑肌细胞功能受损。IdB 1056对微血管通透性增加和白细胞黏附于微静脉血管具有抑制作用。事实上，在预先用或未用环氧化酶抑制剂吲哚美辛预处理的糖尿病仓鼠中，用IdB 1056治疗可恢复对Ach和SNP的舒张反应。总之，在体内微循环水平观察到的IdB 1056的作用可防止与糖尿病和/或氧化应激相关的内皮细胞功能损伤。
