Démolis N, Mallet L, Bussereau F, Jacquet M
Université Paris-Sud Orsay, France.
Biotechniques. 1995 Mar;18(3):453-7.
Large-scale DNA sequencing projects require the use of specific and reliable strategies. Here, we describe an improved strategy using DNaseI cleavage and sequencing strategy using DNaseI cleavage and sequencing from both ends of the plasmid inserts. This strategy yields better results than those obtained using partial digestion with restriction enzymes and cloning in single-stranded vectors.
