Tettelin H, Thierry A, Goffeau A, Dujon B
Département des Biotechnologies (URA 1300 du CNRS and UFR927 Université P.M. Curie), Institut Pasteur, Paris, France.
Yeast. 1998 May;14(7):601-16. doi: 10.1002/(SICI)1097-0061(199805)14:7<601::AID-YEA262>3.0.CO;2-R.
The high resolution complete physical maps of chromosomes VII and XV were constructed to form the basis for sequencing these chromosomes as part of the European systematic sequencing programme of the yeast genome, using a unique cosmid library from strain FY1679, and an original top-down mapping strategy involving I-Sce I chromosome fragmentation. A total of 138 and 196 cosmid clones were used to construct the maps for VII and XV, respectively, forming two unique contigs that cover the entirety of chromosomes (1091 kb each), except the telomeric repeats. Colinearity of the cosmid inserts with yeast DNA was verified, and the physical maps were eventually compared with the independently generated genetic maps.
构建了VII号和XV号染色体的高分辨率完整物理图谱，作为酵母基因组欧洲系统测序计划的一部分，为这些染色体的测序奠定基础。使用来自FY1679菌株的独特粘粒文库，以及涉及I-Sce I染色体片段化的原始自上而下的作图策略。分别使用138个和196个粘粒克隆构建VII号和XV号染色体的图谱，形成两个独特的重叠群，覆盖了除端粒重复序列外的整个染色体（每条染色体1091 kb）。验证了粘粒插入片段与酵母DNA的共线性，并最终将物理图谱与独立生成的遗传图谱进行了比较。
