Honess D J, Andrews M S, Ward R, Bleehen N M
MRC Unit, MRC Centre, Cambridge, U.K.
Acta Oncol. 1995;34(3):385-9. doi: 10.3109/02841869509093994.
The time course and dose response of 600 mm3 subcutaneous RIF-1 tumours to pentoxifylline was measured in terms of relative tumour perfusion, assayed by 86Rb extraction, and pO2 distribution measured by the Eppendorf histograph. Both perfusion and pO2 distribution were maximally increased by 20 mg/kg pentoxifylline 15 min after administration, perfusion to 141 +/- 15% (2 SE) of control, and median pO2 from 3 to 15 mmHg, with the percentage of values < 2.5 mmHg falling from 44 +/- 8% to 22 +/- 7%. Fifteen minutes after administration the pO2 increases were linearly dose-dependent up to 20 mg/kg. Correlation coefficients for perfusion data with the percentages of low values were, for time course and dose response data respectively, 0.76 and 0.84 for median pO2, 0.84 and 0.97 for percentage < 2.5 mmHg and 0.81 and 0.87 for percentage < 10 mmHg. The data show good correlation between changes in perfusion and pO2 distribution parameters, with better correlation for percentage of low values than for median pO2.
通过86Rb提取法测定相对肿瘤灌注，并通过Eppendorf组织氧张力仪测量pO2分布，以此来衡量体积为600 mm3的皮下RIF-1肿瘤对己酮可可碱的时间进程和剂量反应。给药后15分钟，20 mg/kg己酮可可碱可使灌注和pO2分布均达到最大增加，灌注增加至对照的141±15%（2个标准误），中位pO2从3 mmHg升至15 mmHg，<2.5 mmHg值的百分比从44±8%降至22±7%。给药后15分钟，pO2的增加在剂量高达20 mg/kg时呈线性剂量依赖性。灌注数据与低值百分比的相关系数，时间进程和剂量反应数据中，中位pO2分别为0.76和0.84，<2.5 mmHg百分比分别为0.84和0.97，<10 mmHg百分比分别为0.81和0.87。数据表明灌注变化与pO2分布参数之间具有良好的相关性，低值百分比的相关性优于中位pO2。
