Girsh E, Greber Y, Meidan R
Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Biol Reprod. 1995 Apr;52(4):954-62. doi: 10.1095/biolreprod52.4.954.
Endothelial cells, the most abundant cell type in the bovine CL, were shown to establish intercellular contact with steroidogenic cells of the CL. Two experimental models were used to study the involvement of endothelial cells in luteal cell function: 1) luteal slices in which the integrity and communication between the different cells were maintained and 2) pure large and small luteal-like cells, cultured separately or co-cultured with endothelial cells. The luteolytic effect of prostaglandin (PG) F2 alpha was examined in these two models. Treatment with PGF2 alpha did not alter P4 secretion stimulated by LH in young (2-4-day-old) CL slices, whereas, in slices from mature (6-12 days old) CL, PGF2 alpha significantly reduced (by 40%) the stimulatory effect of LH on P4 secretion. In pure large luteal-like cells, the effect of forskolin plus PGF2 alpha on P4 secretion did not differ from forskolin given alone after 3 or 24 h of incubation. However, when co-cultured with endothelial cells, PGF2 alpha significantly inhibited forskolin stimulation. Endothelial cells significantly stimulated P4 production from large luteal-like cells only. This effect may be attributed to the action of PGI2 secreted by endothelial cells. In summary, endothelial cells may play an essential role in luteal functions by being involved in both luteotrophic and luteolytic processes.
内皮细胞是牛黄体中最丰富的细胞类型，已证明其能与黄体的类固醇生成细胞建立细胞间联系。使用了两种实验模型来研究内皮细胞在黄体细胞功能中的作用：1）黄体切片，其中不同细胞之间的完整性和通讯得以维持；2）纯的大、小黄体样细胞，单独培养或与内皮细胞共培养。在这两种模型中检测了前列腺素（PG）F2α的黄体溶解作用。用PGF2α处理不会改变年轻（2 - 4日龄）黄体切片中LH刺激的P4分泌，然而，在成熟（6 - 12日龄）黄体的切片中,PGF2α显著降低（40%）了LH对P4分泌的刺激作用。在纯的大黄体样细胞中，孵育3小时或24小时后，福斯可林加PGF2α对P4分泌的作用与单独给予福斯可林没有差异。然而，当与内皮细胞共培养时，PGF2α显著抑制福斯可林刺激。内皮细胞仅显著刺激大黄体样细胞的P4产生。这种作用可能归因于内皮细胞分泌的PGI2的作用。总之，内皮细胞可能通过参与黄体营养和黄体溶解过程在黄体功能中发挥重要作用。
