Rekawiecki Robert, Wrobel Michal Hubert, Zajac Paulina, Serej Oliwia, Kowalik Magdalena Karolina
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland.
Animals (Basel). 2023 Aug 31;13(17):2784. doi: 10.3390/ani13172784.
The aims of this study were to examine the effect of luteotropic and luteolytic factors on the mRNA and protein expression of the coactivators HAT: histone acetyltransferase p300 (P300), cyclic adenosine monophosphate response element-binding protein (CREB), and steroid receptor coactivator-1 (SRC-1) and the corepressor: nuclear receptor corepressor-2 (NCOR-2) in bovine luteal cells on days 6-10 and 16-20. HAT and HDAC activities were also measured. The obtained results showed that luteotropic and luteolytic factors influence changes in the mRNA and protein levels of the coregulators of PGRs. However, they did not affect the activity of related HAT and HDAC, respectively. Therefore, it is possible that these factors, through changes in the expression of nuclear receptor coactivators and corepressors, may affect the functioning of the nuclear receptors, including PGRs, in the bovine CL.
本研究的目的是检测促黄体生成因子和溶黄体因子对第6 - 10天和16 - 20天牛黄体细胞中辅激活因子组蛋白乙酰转移酶（HAT）：组蛋白乙酰转移酶p300（P300）、环磷酸腺苷反应元件结合蛋白（CREB）和类固醇受体辅激活因子-1（SRC-1）以及辅抑制因子核受体辅抑制因子-2（NCOR-2）的mRNA和蛋白表达的影响。还测定了HAT和HDAC活性。所得结果表明，促黄体生成因子和溶黄体因子影响孕激素受体（PGRs）共调节因子的mRNA和蛋白水平变化。然而，它们分别不影响相关HAT和HDAC的活性。因此，这些因子可能通过核受体辅激活因子和辅抑制因子表达的变化，影响牛黄体中包括PGRs在内的核受体的功能。
