Lin Q, Yao Z
First Affiliated Hospital, Chongqing University of Medical Sciences.
Zhonghua Yi Xue Za Zhi. 1995 Mar;75(3):136-8, 188.
Acute hemorrhagic necrotizing pancreatitis (AHNP) was induced by introduct retrograde injection of the autologous bile in canine. The cryoprobe was under the influence of temperature ranging from -160C to -190C for fifteen to eighteen seconds, and the frozen areas were about 80% surface of pancreas. Cryotherapy of AHNP strongly inhibited the development of the inflammation in pancreatic parenchyma. Postcryotherapy 24hr for remarkably decreased the activities of pancreatic enzymes. The activities of phospholipase A, lipase and amylase in serum decreased by 502.1%, 355.8%, 502.5% respectively in 7 days. The level of blood glucose decreased. At the second week, the fibroblasts were growing around the residual pancreatic aciner cells and insular cells rich in intact zymogen granules. The mortality (6.89%) of the treatment group within 48 hours was significantly lower than that of the control group (100%).
通过向犬逆行注射自体胆汁诱导急性出血性坏死性胰腺炎（AHNP）。冷冻探头在-160℃至-190℃的温度下作用15至18秒，冷冻区域约为胰腺表面的80%。AHNP的冷冻疗法强烈抑制胰腺实质炎症的发展。冷冻治疗24小时后，胰腺酶的活性显著降低。血清中磷脂酶A、脂肪酶和淀粉酶的活性在7天内分别下降了502.1%、355.8%、502.5%。血糖水平下降。在第二周，成纤维细胞在残留的胰腺腺泡细胞和富含完整酶原颗粒的胰岛细胞周围生长。治疗组48小时内的死亡率（6.89%）显著低于对照组（100%）。
