Manteuffel-Cymborowska M, Chmurzyńska W, Peska M, Grzelakowska-Sztabert B
Nencki Institute of Experimental Biology, Warsaw, Poland.
Int J Biochem Cell Biol. 1995 Mar;27(3):287-95. doi: 10.1016/1357-2725(94)00070-r.
Administration of testosterone to female mice causes hypertrophy of their kidneys with spectacular induction of ornithine decarboxylase and significant increase in the level of putrescine. We tried to find out whether testosterone treatment affects also the renal activities of enzymes participating in the formation and utilization of ornithine, specifically arginase and ornithine aminotransferase, and whether they are dependent on putrescine level. Swiss, CFW, DBA2 or F1 (CFW x DBA2) female and male mice were injected with testosterone (125 mg/kg) or CB 3717 (100 mg/kg). DFMO was applied in the drinking water. The activities of the enzymes were determined 24 hr or 5 days after administration of CB 3717 or testosterone, respectively. Renal activities of ornithine decarboxylase (ODC), arginase and ornithine aminotransferase (OAT) were found to be sex-differentiated. The highest activity of ODC was characteristic for the kidneys of males, whereas those of arginase and OAT for the kidneys of females. In the kidneys of testosterone-treated female mice a decrease (50%) of OAT, and a significant increase of arginase activities (up to 200%), were observed. In the males these changes were less pronounced. DFMO, which completely inhibited the activity of renal ODC, did not influence significantly the testosterone-induced arginase and the testosterone-decreased OAT. Arginase and OAT, in contrast to ODC, were not changed in CB 3717-induced hyperplastic kidney. The study showed testosterone-induced differential changes in the activity of two enzymes involved in ornithine biosynthesis and catabolism which accompanied ODC induction in female mouse kidney.(ABSTRACT TRUNCATED AT 250 WORDS)
给雌性小鼠注射睾酮会导致其肾脏肥大，同时显著诱导鸟氨酸脱羧酶，并使腐胺水平大幅升高。我们试图探究睾酮治疗是否也会影响参与鸟氨酸形成和利用的酶的肾脏活性，特别是精氨酸酶和鸟氨酸转氨酶，以及它们是否依赖于腐胺水平。给瑞士、CFW、DBA2或F1（CFW×DBA2）品系的雌性和雄性小鼠注射睾酮（125毫克/千克）或CB 3717（100毫克/千克）。在饮用水中添加二氟甲基鸟氨酸（DFMO）。分别在注射CB 3717或睾酮后24小时或5天测定酶的活性。发现肾脏中鸟氨酸脱羧酶（ODC）、精氨酸酶和鸟氨酸转氨酶（OAT）的活性存在性别差异。ODC的最高活性是雄性小鼠肾脏的特征，而精氨酸酶和OAT的最高活性则是雌性小鼠肾脏的特征。在经睾酮处理的雌性小鼠肾脏中，观察到OAT活性降低（50%），精氨酸酶活性显著增加（高达200%）。在雄性小鼠中，这些变化不太明显。完全抑制肾脏ODC活性的DFMO，对睾酮诱导的精氨酸酶和睾酮降低的OAT没有显著影响。与ODC不同，在CB 3717诱导的增生性肾脏中，精氨酸酶和OAT没有变化。该研究表明，睾酮诱导了雌性小鼠肾脏中参与鸟氨酸生物合成和分解代谢的两种酶的活性发生差异变化，这些变化与ODC的诱导同时发生。（摘要截断于250字）
