Manteuffel-Cymborowska M, Chmurzyńska W, Peska M, Grzelakowska-Sztabert B
Nencki Institute of Experimental Biology, Warsaw, Poland.
Mol Cell Biochem. 1997 Mar;168(1-2):51-7. doi: 10.1023/a:1006834525822.
The presence of a tumour significantly changes nitrogen metabolism, including that of amino acids and polyamines, in host animals. In this study, we examine whether developing tumours affect the metabolic relationship of arginine and ornithine, precursors of polyamines, in the testosterone-induced hypertrophic mouse kidney model. Androgen-induced changes in the activity of enzymes involved with ornithine biosynthesis (arginase), its consumption (ornithine aminotransferase, OAT and ornithine decarboxylase, ODC) and the hypertrophy of host mouse kidney were not affected by the presence of an ascitic tumour (EAC) and only slightly by a mammary carcinoma (MaCa). The HPLC determined renal level of arginine and ornithine showed a striking homeostasis and was disturbed neither by testosterone nor EAC. The effect of MaCa and testosterone on the levels of both amino acids, although significant, was not very pronounced. Developing tumours, especially ascitic, altered the renal activity of OAT and ODC, but not of arginase, in testosterone-untreated mice. All examined tumours, EAC, L 1210 and MaCa actively metabolized arginine and ornithine. the tumour content of arginine which coincided with the activity of arginase, resulted in a marked increase of the ornithine/arginine ratio in tumours, when compared with kidneys. These results indicate that the androgen-induced anabolic response in mouse kidney is preserved, in spite of tumour requirements for essential metabolites.
肿瘤的存在会显著改变宿主动物体内的氮代谢，包括氨基酸和多胺的代谢。在本研究中，我们在睾酮诱导的肥大小鼠肾脏模型中，研究正在生长的肿瘤是否会影响多胺前体精氨酸和鸟氨酸的代谢关系。雄激素诱导的与鸟氨酸生物合成相关的酶（精氨酸酶）活性变化、其消耗（鸟氨酸转氨酶，OAT和鸟氨酸脱羧酶，ODC）以及宿主小鼠肾脏肥大，不受腹水瘤（EAC）存在的影响，仅受乳腺癌（MaCa）轻微影响。高效液相色谱法测定的肾脏精氨酸和鸟氨酸水平显示出显著的稳态，既不受睾酮也不受EAC的干扰。MaCa和睾酮对两种氨基酸水平的影响虽然显著，但并不十分明显。在未用睾酮处理的小鼠中，正在生长的肿瘤，尤其是腹水瘤，改变了OAT和ODC的肾脏活性，但未改变精氨酸酶的活性。所有检测的肿瘤，EAC、L 1210和MaCa均积极代谢精氨酸和鸟氨酸。与肾脏相比，与精氨酸酶活性一致的肿瘤精氨酸含量导致肿瘤中鸟氨酸/精氨酸比值显著增加。这些结果表明，尽管肿瘤需要必需代谢物，但小鼠肾脏中雄激素诱导的合成代谢反应仍得以保留。
