Choi-Lundberg D L, Bohn M C
Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
Brain Res Dev Brain Res. 1995 Mar 16;85(1):80-8. doi: 10.1016/0165-3806(94)00197-8.
Glial cell line-derived neurotrophic factor (GDNF) is a member of the transforming growth factor-beta family isolated from the rat glial tumor cell line, B49. In embryonic dopaminergic (DA) neurons in vitro, GDNF promotes survival, high-affinity dopamine uptake, and neurite outgrowth. We have used a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) with primers specific to GDNF to study the developmental expression of GDNF mRNA in central nervous system (CNS) and peripheral organs of embryonic rat on gestational days E11.5, E13.5 and E18, neonatal rat on postnatal days P0 and P10, and adult rat. GDNF mRNA is expressed throughout the CNS, with highest levels in P0 spinal cord and in P0 and P10 striatum. Lower levels are present in the brainstem (including the ventral mesencephalon, which contains the DA neurons of the substantia nigra), cerebellum, diencephalon, and telencephalon, as well as in primary cultures of cerebellar granule cells prepared from P7 cerebellum and astrocytes prepared from P1 cortex. The cerebellum has an unusual temporal pattern of expression, high at birth and in the adult, but undetectable at P10. GDNF mRNA is also expressed in many peripheral tissues at higher levels than in brain. These include embryonic limb bud, kidney and gut; neonatal kidney, gut, lung and testis; and adult lung, liver and ovary. In addition to the predicted RT-PCR product, we also observed a minor band which was shown to be identical to GDNF in the mature peptide sequence, but which has a 78 base pair deletion in the preproprotein sequence.(ABSTRACT TRUNCATED AT 250 WORDS)
胶质细胞系源性神经营养因子（GDNF）是从大鼠胶质细胞瘤细胞系B49中分离出的转化生长因子-β家族的一员。在体外培养的胚胎多巴胺能（DA）神经元中，GDNF可促进其存活、高亲和力摄取多巴胺以及神经突生长。我们使用了针对GDNF的特异性引物进行半定量逆转录聚合酶链反应（RT-PCR），以研究妊娠第E11.5、E13.5和E18天的胚胎大鼠、出生后第P0和P10天的新生大鼠以及成年大鼠的中枢神经系统（CNS）和外周器官中GDNF mRNA的发育表达情况。GDNF mRNA在整个CNS中均有表达，在P0脊髓以及P0和P10纹状体中的表达水平最高。在脑干（包括含有黑质DA神经元的腹侧中脑）、小脑、间脑、端脑以及从P7小脑制备的小脑颗粒细胞原代培养物和从P1皮质制备的星形胶质细胞中表达水平较低。小脑具有不寻常的表达时间模式，出生时和成年时表达水平高，但在P10时无法检测到。GDNF mRNA在许多外周组织中的表达水平也高于大脑。这些组织包括胚胎肢芽、肾脏和肠道；新生肾脏、肠道、肺和睾丸；以及成年肺、肝脏和卵巢。除了预测的RT-PCR产物外，我们还观察到一条小条带，其成熟肽序列与GDNF相同，但前体蛋白序列中有78个碱基对的缺失。（摘要截短于250字）
