Jaszai J, Farkas L, Galter D, Reuss B, Strelau J, Unsicker K, Krieglstein K
Neuroanatomy, University of Heidelberg, Germany.
J Neurosci Res. 1998 Aug 15;53(4):494-501. doi: 10.1002/(SICI)1097-4547(19980815)53:4<494::AID-JNR12>3.0.CO;2-2.
Persephin (PSP) is the most recently discovered member of the GDNF family of neurotrophic factors. We have used an RT-PCR approach to start addressing the putative functional significance of PSP by determining sites of its synthesis in the neonatal rat brain. Generally, two transcripts were found. Sequence analysis of the transcripts identifies an 88 bp intronic sequence. Neural tissues analysed included cortex, hippocampus, striatum, diencephalon, mesencephalon, cerebellum, hindbrain and spinal cord as well as superior cervical, dorsal root ganglia, adrenal gland, and PC12 pheochromocytoma cells. As non-neuronal tissues, sciatic nerve, optic nerve, primary astroglial, oligodendroglial, O2A progenitor, and glioma cells (C6, B49) were also included. All tissues/cells except oligodendrocytes and O2A progenitor cells were strongly positive for PSP mRNA. To test the hypothesis of whether PSP might act as a target-derived factor, as suggested for GDNF, the motoneuron-muscle axis has been analysed. PSP is synthesized in skeletal muscle and, to a higher extent, in the spinal cord. Moreover, PSP is synthesized in purified embryonic motoneurons. Together, these data do not support a role for PSP as a typical target-derived neurotrophic factor for motoneurons. We conclude that PSP is synthesized throughout the nervous system and that it is presumably of both astroglial and neuronal origin, in contrast to GDNF and neurturin, which seem to be predominantly of neuronal origin.
Persephin（PSP）是神经营养因子GDNF家族中最近发现的成员。我们采用逆转录聚合酶链反应（RT-PCR）方法，通过确定PSP在新生大鼠脑中的合成位点，开始探讨其假定的功能意义。一般来说，发现了两种转录本。对转录本的序列分析确定了一个88bp的内含子序列。分析的神经组织包括皮质、海马、纹状体、间脑、中脑、小脑、后脑和脊髓，以及颈上神经节、背根神经节、肾上腺和PC12嗜铬细胞瘤细胞。作为非神经组织，还包括坐骨神经、视神经、原代星形胶质细胞、少突胶质细胞、O2A祖细胞和胶质瘤细胞（C6、B49）。除少突胶质细胞和O2A祖细胞外，所有组织/细胞的PSP mRNA均呈强阳性。为了验证PSP是否可能像GDNF那样作为一种靶源性因子起作用的假设，对运动神经元-肌肉轴进行了分析。PSP在骨骼肌中合成，在脊髓中合成程度更高。此外，PSP在纯化的胚胎运动神经元中合成。总之，这些数据不支持PSP作为运动神经元典型的靶源性神经营养因子的作用。我们得出结论，PSP在整个神经系统中合成，推测其起源于星形胶质细胞和神经元，这与GDNF和神经营养因子不同，后者似乎主要起源于神经元。
