Nuutinen H, Ginanni Corradini S, Jüngst D, Lange V, Abei M, Schwarzendrube J, Williams C, Holzbach R T
Gastrointestinal Research Unit, Research Institute, Cleveland Clinic Foundation, Ohio 44195-5218, USA.
Dig Dis Sci. 1995 Jun;40(6):1174-8. doi: 10.1007/BF02065520.
A biliary form of the alpha 1-acid glycoprotein (AAG) promotes cholesterol crystallization in the lower-molecular-weight, concanavalin A-bound fraction of gallbladder bile. In addition, bile AAG concentration is higher in cholesterol gallstone patients with multiple stones than in control patients without gallstone disease. In this study we sought to determine whether the increased biliary concentration of AAG in cholesterol gallstone patients is accompanied by a more rapid nucleation time in patients with multiple stones. AAG concentration in native biles was measured by ELISA. Nucleation time was measured using a standard microscopy method. The concentration of biliary AAG was then related to nucleation time in biles from the same patients. Nucleation times were significantly shorter (< or = 5 days) in cholesterol gallstone patients with raised AAG concentrations (P < 0.03). There was a significant (P = 0.004) negative correlation (r = -0.53) between nucleation time and the AAG concentration in cholesterol gallstone patients with multiple stones. The concentration of biliary AAG appears to exert an important influence on the speed of cholesterol nucleation in bile in many patients with cholesterol gallstone disease.
α1-酸性糖蛋白（AAG）的一种胆汁形式可促进胆囊胆汁中低分子量、伴刀豆球蛋白A结合部分的胆固醇结晶。此外，与无胆结石疾病的对照患者相比，患有多发性结石的胆固醇胆结石患者胆汁中AAG浓度更高。在本研究中，我们试图确定胆固醇胆结石患者胆汁中AAG浓度升高是否伴随着多发性结石患者更短的成核时间。通过酶联免疫吸附测定法（ELISA）测量天然胆汁中AAG的浓度。使用标准显微镜方法测量成核时间。然后将胆汁中AAG的浓度与同一患者胆汁中的成核时间相关联。AAG浓度升高的胆固醇胆结石患者的成核时间明显更短（≤5天）（P<0.03）。在患有多发性结石的胆固醇胆结石患者中，成核时间与AAG浓度之间存在显著的（P = 0.004）负相关（r = -0.53）。胆汁中AAG的浓度似乎对许多胆固醇胆结石疾病患者胆汁中胆固醇成核的速度有重要影响。
