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[屠宰育肥猪血清和骨骼中钙、磷、镁的矿物质含量及血清碱性磷酸酶活性]

[The mineral content of calcium, phosphorus and magnesium in the serum and bones and the serum activity of alkaline phosphatase in slaughtered fattening pigs].

作者信息

Prikoszovits A, Schuh M

机构信息

II. Medizinischen Universitätsklinik für Klauentiere, Veterinärmedizinischen Universität Wien.

出版信息

Dtsch Tierarztl Wochenschr. 1995 Jan;102(1):53-5.

PMID:7781541
Abstract

The mineral content of serum and bones in 311 fattening pigs kept in a large scale farming operation was analyzed. Serum and metacarpal (III and IV) bone samples were obtained during slaughtering. The mean values of serum parameters as determined by automated dry-chemistry (Kodak Ektachem) were: Ca 2.80 +/- 0.20 mmol/l, P 3.08 +/- 0.67 mmol/l, Mg 1.21 +/- 0.24 mmol/l and Alkaline Phosphatase 211.25 +/- 56.91 U/l. Ash in bones was on average 62.85 +/- 2.07%. Ca- und Mg-contents of bones were analyzed by atomic absorption spectrophotometry, the percentage found for Ca was 31.87 +/- 2.68%, and for Mg 0.64 +/- 0.05%. P in bones was determined spectrophotometrically by the vanadate-molybdate method and was 17.48 +/- 0.97%. The Ca/P-ratio in serum was 0.94 +/- 0.16, in bones 1.83 +/- 0.19, Ca/Mg in serum 2.37 +/- 0.31 and in bones 50.05 +/- 5.00. The parameters tested (Ca, P, Mg, AP) and the Ca/P- and Ca/Mg-ratios of minerals in serum and bones did not differ between sexes. Between all serum parameters positive correlations were found as well as between Ca and Mg in bones. Ca, P and Mg in bones were negatively correlated to Ca, P and Mg in serum, respectively. All results of the parameters examined were within the normal range for healthy fattening pigs, with the only exception that Ca-values in bones were lower than normal. A new method for preparing the bone samples for the analyses of minerals was established and can be recommended for further investigations.

摘要

对大规模养殖的311头育肥猪的血清和骨骼矿物质含量进行了分析。在屠宰过程中采集血清和掌骨(第三和第四掌骨)骨样本。通过自动干化学法(柯达Ektachem)测定的血清参数平均值为:钙2.80±0.20毫摩尔/升,磷3.08±0.67毫摩尔/升,镁1.21±0.24毫摩尔/升,碱性磷酸酶211.25±56.91 U/升。骨骼中的灰分平均为62.85±2.07%。采用原子吸收分光光度法分析骨骼中的钙和镁含量,钙的含量为31.87±2.68%,镁的含量为0.64±0.05%。采用钒酸盐-钼酸盐法分光光度法测定骨骼中的磷含量,为17.48±0.97%。血清中的钙/磷比值为0.94±0.16,骨骼中的钙/磷比值为1.83±0.19,血清中的钙/镁比值为2.37±0.31,骨骼中的钙/镁比值为50.05±5.00。所测试的参数(钙、磷、镁、碱性磷酸酶)以及血清和骨骼中矿物质的钙/磷和钙/镁比值在性别之间没有差异。在所有血清参数之间以及骨骼中的钙和镁之间发现了正相关。骨骼中的钙、磷和镁分别与血清中的钙、磷和镁呈负相关。所有检测参数的结果均在健康育肥猪的正常范围内,唯一例外是骨骼中的钙值低于正常水平。建立了一种用于分析矿物质的骨骼样本制备新方法,可推荐用于进一步研究。

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