Dołegowska Barbara
Zakładu Biochemii i Chemii Pomorskiej Akademii Medycznej w Szczecinie, al. Powstańców Wlkp. 72, 70-111 Szczecin.
Ann Acad Med Stetin. 2002;48:45-58.
The study was done in Starbro chicks aged 1 to 50 days. Two chicks were collected every day and the femoral bones were removed. Altogether, 200 bones were studied. The material was divided into 5 age groups (I to V). Compact and spongy bone, bone marrow and articular cartilage were sampled. Lipids were extracted according to Folch et al. Total lipids were measured spectrophotometrically. Gas chromatography was used to study the content of individual fatty acids. Calcium (Ca), phosphorus (P), magnesium (Mg) and zinc (Zn) levels were determined using atomic absorption spectrometry (Philips PU 9100X), while fluorine content was studied with gas chromatography. As the distribution of the results deviated from normal, non-parametric Kruskal-Wallis and median tests were used. Correlations were studied with Spearman's Rs coefficients. Computations were done with Statistica 5.0 (StatSoft) software and the level of significance was taken as p < 0.05. Statistically significant differences in the fatty acid profile (Tab. 1) and content of elements (Tab. 2) were found. The content of fatty acids, calcium and fluorine correlated with age. Fatty acids with the highest content in bone tissues were C18:1, C16:0, C18:2 and C18:0. The highest content of fatty acids was found in bone marrow and the lowest in articular cartilage. Several correlations were revealed between individual fatty acids and elements (Tab. 3, 4). The following conclusions were drawn: 1) The fatty acid profile in compact and spongy bone, bone marrow and articular cartilage changed according to age of chicks; 2) Content of calcium, phosphorus, magnesium, zinc and fluoride correlated with age; 3) Oleic (C18:1), palmitic (C16:0), linoleic (C18:2), stearic (C18:0) and arachidonic acid (C20:4) accounted for the majority of the fatty acid pool; 4) Correlations in the content of fatty acids and elements were noted between bone structures. Fatty acids appear to play an important role in the metabolism of bone tissues, particularly in mineralization processes.
该研究在1至50日龄的星布罗雏鸡上进行。每天收集两只雏鸡并取出股骨。总共研究了200根骨头。材料被分为5个年龄组（I至V）。对密质骨、松质骨、骨髓和关节软骨进行采样。按照福尔奇等人的方法提取脂质。用分光光度法测量总脂质。用气相色谱法研究单个脂肪酸的含量。使用原子吸收光谱仪（飞利浦PU 9100X）测定钙（Ca）、磷（P）、镁（Mg）和锌（Zn）的水平，而用气相色谱法研究氟含量。由于结果分布偏离正态，使用非参数克鲁斯卡尔 - 沃利斯检验和中位数检验。用斯皮尔曼Rs系数研究相关性。使用Statistica 5.0（StatSoft）软件进行计算，显著性水平取为p < 0.05。发现脂肪酸谱（表1）和元素含量（表2）存在统计学显著差异。脂肪酸、钙和氟的含量与年龄相关。骨组织中含量最高的脂肪酸是C18:1、C16:0、C18:2和C18:0。脂肪酸含量在骨髓中最高，在关节软骨中最低。单个脂肪酸与元素之间发现了几种相关性（表3、4）。得出以下结论：1）密质骨、松质骨、骨髓和关节软骨中的脂肪酸谱随雏鸡年龄而变化；2）钙、磷、镁、锌和氟的含量与年龄相关；3）油酸（C18:1）、棕榈酸（C16:0）、亚油酸（C18:2）、硬脂酸（C18:0）和花生四烯酸（C20:4）占脂肪酸池的大部分；4）在骨结构之间注意到脂肪酸和元素含量的相关性。脂肪酸似乎在骨组织代谢中起重要作用，特别是在矿化过程中。
