Cooper A D, Creaser C S, Farrington W H, Tarbin J A, Shearer G
Ministry of Agriculture, Fisheries and Food, Food Science Laboratory, Colney, Norwich, UK.
Food Addit Contam. 1995 Mar-Apr;12(2):167-76. doi: 10.1080/02652039509374291.
The application of solid-phase extraction and HPLC with UV-diode array detection to the multi-residue determination of veterinary drugs is described. A two-stage SPE clean-up was employed, using C18 and silica cartridges. HPLC analysis was carried out on a base-deactivated C8 column using gradient elution systems at two pH values. The procedure establishes the basis of a method for routine screening of pig kidney samples for some sulphonamides, benzimidazoles, nitroimidazoles and nitrofurans at concentrations at or below the UK Maximum Residue Limits (MRLs). Limits of detection of 2-18 micrograms/kg could be achieved for these analytes at recoveries of 40-70%. UV spectra measured on-line were used for confirmation of peak identities at these concentrations. The possibility of extension of this procedure to a wider range of analyses is discussed.
