Kodama J, Ryoji H, Okuda H, Yoshinouchi M, Kudo T
Department of Obstetrics and Gynecology, Okayama University Medical School, Japan.
Eur J Obstet Gynecol Reprod Biol. 1995 Mar;59(1):45-51. doi: 10.1016/0028-2243(94)02024-9.
Immunohistochemical study was employed using a monoclonal antibody (C219) to investigate P-glycoprotein expression in 23 normal endometria and 40 endometrial adenocarcinomas. P-glycoprotein immunopositivity was observed in the mid- to late-proliferative phase and the whole secretory phase of normal endometrium. In contrast, no P-glycoprotein was detected in endometrium of early proliferative phase nor post-menopausal endometrium. We also investigated the expression of P-glycoprotein in endometrial cancers to find 16 out of the 40 endometrial adenocarcinomas (40%) expressed P-glycoprotein. P-glycoprotein immunopositivity was often observed in gland growing parts but not in solid growing parts in a given tumor specimen. Proliferative activity of the tumor, as measured by PCNA labeling, was significantly higher in the P-glycoprotein-negative group than that in P-glycoprotein-positive group (P < 0.005). Thus, there was a strong reverse relation between P-glycoprotein expression and proliferative activity in endometrial adenocarcinomas.
采用免疫组织化学方法，使用单克隆抗体（C219）研究23例正常子宫内膜和40例子宫内膜腺癌中P-糖蛋白的表达情况。在正常子宫内膜的增殖中期至晚期以及整个分泌期观察到P-糖蛋白免疫阳性。相比之下，在增殖早期子宫内膜和绝经后子宫内膜中未检测到P-糖蛋白。我们还研究了子宫内膜癌中P-糖蛋白的表达情况，发现40例子宫内膜腺癌中有16例（40%）表达P-糖蛋白。在给定的肿瘤标本中，P-糖蛋白免疫阳性常见于腺体生长部位，而非实体生长部位。通过PCNA标记测量的肿瘤增殖活性，在P-糖蛋白阴性组中显著高于P-糖蛋白阳性组（P < 0.005）。因此，子宫内膜腺癌中P-糖蛋白表达与增殖活性之间存在强烈的负相关关系。
