Aerne B L, Baader C D, Schmid V
Institute of Zoology, University of Basel, Switzerland.
Dev Biol. 1995 Jun;169(2):547-56. doi: 10.1006/dbio.1995.1168.
We have isolated from the hydrozoan Podocoryne carnea a genomic clone and the corresponding cDNA of a homeobox gene. This gene, called cnox1-Pc, is most closely related to the lab class of homeobox genes. In the life cycle of P. carnea, which involves an asexually reproducing, sedentary polyp and a sexual planktonic medusa stage, we found cnox1-Pc transcripts specifically expressed in the medusa stage. Expression studies on isolated medusa organs and tissues indicate that the message is absent in the tentacles and the feeding and sexual organ of the medusa and present in the striated muscle cells. During medusa bud development, expression of cnox1-Pc can be correlated with the development of the striated muscle tissue, a cell type only found in the medusa. The results suggest that cnox1-Pc is involved in pattern formation of the medusa of P. carnea and might have a regulatory role in the differentiation of striated muscle.
我们从水螅纲动物卡氏柄海鞘中分离出一个基因组克隆以及一个同源异型框基因的相应cDNA。这个基因名为cnox1-Pc，与同源异型框基因的lab类最为密切相关。在卡氏柄海鞘的生命周期中，包括无性繁殖的固着水螅体阶段和有性浮游水母阶段，我们发现cnox1-Pc转录本在水母阶段特异性表达。对分离出的水母器官和组织的表达研究表明，该信息在水母的触手、摄食和性器官中不存在，而在横纹肌细胞中存在。在水母芽发育过程中，cnox1-Pc的表达与横纹肌组织的发育相关，横纹肌组织是仅在水母中发现的一种细胞类型。结果表明，cnox1-Pc参与了卡氏柄海鞘水母的模式形成，并且可能在横纹肌的分化中起调节作用。
