Radice P, Pensotti V, Jones C, Perry H, Pierotti M A, Tunnacliffe A
Department of Pathology, University of Cambridge, United Kingdom.
Genomics. 1995 Mar 1;26(1):101-6. doi: 10.1016/0888-7543(95)80087-3.
A novel human gene, ARCN1, has been identified in chromosome band 11q23.3. It maps approximately 50 kb telomeric to MLL, a gene that is disrupted in a number of leukemia-associated translocation chromosomes. cDNA clones representing ARCN1 hybridize to 4-kb mRNA species present in all tissues tested. Sequencing of cDNAs suggests that at least two forms of mRNA with alternative 5' ends are present within the cell. The mRNA with the longest open reading frame gives rise to a protein of 57 kDa. Although the sequence reported is novel, remarkable similarity is observed with two predicted protein sequences from partial DNA sequences generated by rice (Oryza sativa) and fruit fly (Drosophila melanogaster) genome projects. The degree of sequence conservation is comparable to that observed for highly conserved structural proteins, such as heat shock protein HSP70, and is greater than that of gamma-tubulin and heat shock protein HSP60. A more distant relationship to the group of clathrin-associated proteins suggests a possible role in vesicle structure or trafficking. In view of its ancient pedigree and a potential involvement in cellular architecture, we propose that the ARCN1 protein be named archain.
在染色体11q23.3带中鉴定出一个新的人类基因ARCN1。它定位于MLL基因的端粒大约50 kb处，MLL基因在许多与白血病相关的易位染色体中发生破坏。代表ARCN1的cDNA克隆与所有测试组织中存在的4 kb mRNA种类杂交。cDNA测序表明细胞内至少存在两种具有可变5'端的mRNA形式。具有最长开放阅读框的mRNA产生一种57 kDa的蛋白质。尽管所报道的序列是新的，但与水稻（Oryza sativa）和果蝇（Drosophila melanogaster）基因组计划产生的部分DNA序列预测的两种蛋白质序列具有显著的相似性。序列保守程度与热休克蛋白HSP70等高度保守的结构蛋白相当，并且大于γ-微管蛋白和热休克蛋白HSP60。与网格蛋白相关蛋白组的关系较远，提示其可能在囊泡结构或运输中起作用。鉴于其古老的谱系以及可能参与细胞结构，我们建议将ARCN1蛋白命名为archain。
