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δ-COP和ζ-COP是与网格蛋白相关蛋白同源的两种外被体亚基，参与内质网回收过程。

Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval.

作者信息

Cosson P, Démollière C, Hennecke S, Duden R, Letourneur F

机构信息

Basel Institute for Immunology, Switzerland.

出版信息

EMBO J. 1996 Apr 15;15(8):1792-8.

PMID:8617224
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC450095/
Abstract

Two new thermosensitive yeast mutants defective in retrieval of dilysine-tagged proteins from the Golgi back to the endoplasmic reticulum (ER) were characterized. While both ret2-1 and ret3-1 were defective for ER retrieval, only ret2-1 exhibited a defect in forward ER-to-Golgi transport at the non-permissive temperature. Coatomer (COPI) from both mutants could efficiently bind dilysine motifs in vitro. The corresponding RET2 and RET3 genes were cloned by complementation and found of encode the delta and zeta subunits of coatomer respectively. Both proteins show significant homology to clathrin adaptor subunits. These results emphasize the role of coatomer in retrieval of dilysine-tagged proteins back to the ER, and the similarity between clathrin and coatomer coats.

摘要

我们对两个新的温度敏感型酵母突变体进行了表征，它们在将带有双赖氨酸标签的蛋白质从高尔基体运回内质网（ER）的过程中存在缺陷。虽然ret2-1和ret3-1在ER回收方面都存在缺陷，但只有ret2-1在非允许温度下向前的ER到高尔基体运输中表现出缺陷。来自这两个突变体的外被体蛋白（COPI）在体外都能有效结合双赖氨酸基序。通过互补克隆了相应的RET2和RET3基因，发现它们分别编码外被体蛋白的δ和ζ亚基。这两种蛋白质与网格蛋白衔接子亚基具有显著的同源性。这些结果强调了外被体蛋白在将带有双赖氨酸标签的蛋白质运回ER中的作用，以及网格蛋白和外被体蛋白衣被之间的相似性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/de370385d93a/emboj00008-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/de77ff86ef81/emboj00008-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/0246f862655b/emboj00008-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/3a0661363adb/emboj00008-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/de370385d93a/emboj00008-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/de77ff86ef81/emboj00008-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/0246f862655b/emboj00008-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/3a0661363adb/emboj00008-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2354/450095/de370385d93a/emboj00008-0046-a.jpg

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