Uete T, Matsuo K
Department of Clinical Investigation, Kitano Hospital, Tazuke Kofukai Medical Research Institue, Osaka.
Jpn J Antibiot. 1995 Apr;48(4):553-62.
The in vitro antimicrobial activity of cephamycin, e.g. cefmetazole and cephalosporin, such as cefazolin, cefotiam, cefamandole and cefoperazone, alone and in combination, was studied employing 9 strains of methicillin-sensitive Staphylococcus aureus (MSSA) and 30 strains of methicillin-resistant Staphylococcus aureus (MRSA). Using the checkerboard agar dilution method, strong synergism was demonstrable in a majority of MSSA and MRSA strains for cefmetazole combined with these cephalosporins, with the minimum fractional inhibitory concentration index < or = 0.5. In the presence of a concentration < or = 6.25 micrograms/ml of these cephalosporins in Mueller-Hinton agar medium, the activity of cefmetazole against MRSA was most prominently potentiated by cefotiam, followed by cefamandole, cefazolin and cefoperazone. At a concentration of 12.5 micrograms/ml, cefotiam and cefamandole showed a similar effect in potentiation of cefmetazole activity. In hypertonic agar medium containing 4% NaCl, these synergistic combination effects were reduced. However, the activity of cefmetazole and cefamandole in combination under these conditions was influenced to a lesser extent and more potent than that of other combinations.
研究了头孢美唑（如头孢美唑）和头孢菌素（如头孢唑林、头孢替安、头孢孟多和头孢哌酮）单独及联合使用时对9株甲氧西林敏感金黄色葡萄球菌（MSSA）和30株甲氧西林耐药金黄色葡萄球菌（MRSA）的体外抗菌活性。采用棋盘琼脂稀释法，对于头孢美唑与这些头孢菌素联合使用的情况，大多数MSSA和MRSA菌株表现出强协同作用，最小抑菌浓度分数指数≤0.5。在Mueller-Hinton琼脂培养基中这些头孢菌素浓度≤6.25微克/毫升时，头孢替安对头孢美唑抗MRSA的活性增强作用最显著，其次是头孢孟多、头孢唑林和头孢哌酮。在浓度为12.5微克/毫升时，头孢替安和头孢孟多在增强头孢美唑活性方面表现出相似的效果。在含有4%氯化钠的高渗琼脂培养基中，这些协同组合效应减弱。然而，在这些条件下头孢美唑和头孢孟多联合使用时的活性受影响程度较小，且比其他组合更有效。
