Nishimura T, Nagumo F, Ueda H, Tajima Y, Sano M, Shimamoto Y, Tadano J
Department of Laboratory Medicine, Saga Medical School.
Rinsho Ketsueki. 1995 May;36(5):487-92.
A 69-year-old woman was admitted to our hospital because of leucocytosis and systemic lymphadenopathy. On admission, white blood cell count was 163,000/microliters, most of which consisted of flower-like cells with convoluted nuclei in the peripheral blood. In the abnormal lymphocyte cells surface-marker test by flow cytometry showed that they expressed CD2, CD3, CD4, CD29, CD45RA, and CD38, but not CD8, CD16, and CD25. Serum anti-Human T-lymphotropic virus type-I (HTLV-I) antibody was negative in particle agglutination, enzyme-linked immunosorbent assay (ELISA) and western-blotting assay. HTLV-I proviral DNA in the abnormal lymphocyte cells was not detected by southern blotting hybridization technique. Moreover, HTLV-I provirus was not detected using a polymerase-chain-reaction (PCR). A monoclonal rearrangement of the TCR-beta chain gene was evident by using DNA probe in southern blot hybridization. Because of the rapid progress of the disease, chemotherapy was started immediately after admission. Though, this patient became refractory, and she died about 1 year after admission.
一名69岁女性因白细胞增多和全身淋巴结病入院。入院时，白细胞计数为163,000/微升，外周血中大部分为核呈卷曲状的花状细胞。流式细胞术检测异常淋巴细胞表面标志物显示，它们表达CD2、CD3、CD4、CD29、CD45RA和CD38，但不表达CD8、CD16和CD25。颗粒凝集试验、酶联免疫吸附测定（ELISA）和western印迹法检测血清抗I型人类嗜T淋巴细胞病毒（HTLV-I）抗体均为阴性。Southern印迹杂交技术未检测到异常淋巴细胞中的HTLV-I前病毒DNA。此外，使用聚合酶链反应（PCR）也未检测到HTLV-I前病毒。Southern印迹杂交中使用DNA探针显示TCR-β链基因存在单克隆重排。由于病情进展迅速，入院后立即开始化疗。然而，该患者病情难治，入院后约1年死亡。
