Satoh K, Ozaki Y, Kume S
Department of Clinical and Laboratory Medicine, Yamanashi Medical College.
Rinsho Byori. 1995 May;43(5):426-31.
Platelets play an important role in hemostasis and thrombosis. Evaluation of platelet function provides useful information on the diagnosis and cure of diseases associated with hemostasis and thrombosis. Among various platelet functions, platelet aggregometry is most widely used in clinical application. Platelet aggregation has been assessed by measuring the changes in the optical density of the platelet suspensions. However, this method has several shortcomings such as failure to detect the formation of small aggregates and lack of correlation between aggregate formation and changes in optical density. Thus, we have developed a new method of platelet aggregometry based on light scattering. This device emits laser beams against platelet aggregates and efficiently detects light scattering produced by these aggregates. The intensity and frequency of light scattering provides information as to the size and number of aggregates present in a limited area. By this method, we were able to determine several different modes of inhibitory effects on platelet aggregation. The high sensitivity of this method also allowed us to detect slight platelet activation, which had not been possible by the conventional method using changes in optical density. Clinical application of this method revealed frequent occurrence of spontaneous aggregation in diabetic patients and a close relationship between this phenomenon and diabetic complications.
血小板在止血和血栓形成中发挥着重要作用。血小板功能评估为与止血和血栓形成相关疾病的诊断和治疗提供了有用信息。在各种血小板功能中，血小板聚集测定法在临床应用中使用最为广泛。血小板聚集通过测量血小板悬浮液光密度的变化来评估。然而，该方法存在一些缺点，如无法检测小聚集体的形成以及聚集体形成与光密度变化之间缺乏相关性。因此，我们开发了一种基于光散射的新型血小板聚集测定方法。该设备向血小板聚集体发射激光束，并有效检测这些聚集体产生的光散射。光散射的强度和频率提供了关于有限区域内聚集体大小和数量的信息。通过这种方法，我们能够确定几种不同的抑制血小板聚集的模式。该方法的高灵敏度还使我们能够检测到轻微的血小板活化，这是使用光密度变化的传统方法无法做到的。该方法的临床应用显示糖尿病患者中自发聚集频繁发生，并且这种现象与糖尿病并发症之间存在密切关系。
