Ogata K, Ohta K, Kubo H, Yamaguchi A, Watanabe H
Drug Discovery and Research Laboratories, Hoechst Japan Limited, Kawagoe.
Rinsho Byori. 1995 May;43(5):468-71.
Since it was reported that the 64kD antigen that had been found to be the target of autoantibodies in the sera from IDDM patients was the same as GAD, GAD antibodies have been one of the focuses of many investigations. In this study, we developed a sensitive radioimmunoassay (RIA) system for GAD antibodies in human sera using native GAD purified from the pig brain. To confirm the relevance of pig GAD as the target antigen, we compared the sequences and antigenic activities among pig, rat and recombinant human GAD65. Based on the data from our and other laboratories, the homology of amino acid sequences between pig and human GAD65 was 96.4%. The coefficient of correlation between the results of human and pig GAD RIA was 0.948. The GAD antibody study group confirmed the clinical usefulness of GAD antibodies not only for the diagnosis of IDDM but also slowly progressive IDDM.
自从有报道称在胰岛素依赖型糖尿病（IDDM）患者血清中发现的作为自身抗体靶标的64kD抗原与谷氨酸脱羧酶（GAD）相同以来，GAD抗体一直是众多研究的重点之一。在本研究中，我们使用从猪脑中纯化的天然GAD开发了一种用于检测人血清中GAD抗体的灵敏放射免疫分析（RIA）系统。为了确认猪GAD作为靶抗原的相关性，我们比较了猪、大鼠和重组人GAD65之间的序列和抗原活性。根据我们实验室和其他实验室的数据，猪和人GAD65之间的氨基酸序列同源性为96.4%。人源和猪源GAD RIA结果之间的相关系数为0.948。GAD抗体研究组证实了GAD抗体不仅对IDDM的诊断有用，而且对缓慢进展型IDDM也有用。
