Tonken H P, Zhang F, Yim K K, Gencosmanoglu R, Sudekum A E, Campagna-Pinto D, Newlin L, Buncke H J, Lineaweaver W C
Division of Microsurgical Replantation-Transplantation, Davies Medical Center, San Francisco, USA.
Microsurgery. 1995;16(2):98-102. doi: 10.1002/micr.1920160211.
An experimental model for producing venous thrombosis was developed in end-to-end anastomoses of femoral veins in rats. The anastomoses were performed using a suture (9-0 suture-70 microns needle) with a knot 1 cm from the needle. The knot was formed by making either six or eight half-hitches in one throw of the suture. Vessel patency was assessed through the direct "milking test" at 20 min and 24 hrs. The incidence of thrombosis when using one knot with six half-hitches ranged from 20% to 40% and with eight half-hitches, from 50% to 70%. The incidence of femoral vein thrombosis varied directly with the presence and size of the knot. In this model, thrombosis was induced by exaggerating vessel injuries that may occur when performing routine microvascular anastomoses. This study demonstrates a reproducible thrombogenic model which mimics clinical practice and may be used to study the effects of local and systemic antithrombogenic agents.
通过大鼠股静脉端端吻合建立了一种产生静脉血栓形成的实验模型。使用缝线（9-0缝线-70微米针）进行吻合，在距针1厘米处打一个结。通过在缝线的一次缠绕中打六个或八个半结来形成结。在20分钟和24小时时通过直接“挤奶试验”评估血管通畅性。使用一个有六个半结的结时血栓形成的发生率为20%至40%，使用八个半结时为50%至70%。股静脉血栓形成的发生率与结的存在和大小直接相关。在该模型中，血栓形成是通过夸大在进行常规微血管吻合时可能发生的血管损伤而诱导的。本研究展示了一种可重复的血栓形成模型，该模型模拟临床实践，可用于研究局部和全身抗血栓形成药物的作用。
