Nakashima K, Eguchi Y, Nakasone N
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Microbiol Immunol. 1995;39(2):87-94. doi: 10.1111/j.1348-0421.1995.tb02174.x.
A cholera-like enterotoxin was purified from Vibrio cholerae O139 strain AI-1841 isolated from a diarrheal patient in Bangladesh. Its characteristics were compared with that of cholera toxins (CTs) of classical strain 569B and El Tor strain KT25. Al-1841 produced as much toxin as O1 strains. The toxins were indistinguishable in terms of their migration profiles in conventional polyacrylamide gel disc electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectrofocusing as well as their affinity for hydroxyapatite. The skin permeability factor activity and the fluid accumulation induced in rabbit ileal loops of the toxin of AI-1841 were identical to those of the CTs. Three toxins equally reacted against anti-569B CT antiserum in Western blotting, and their B subunits formed a precipitin line against any anti-B subunit antiserum by double gel immunodiffusion. Anti-569B CTB antibody neutralized the three toxins in their PF activities and enterotoxicities. The amino acid sequence of 1841 toxin B subunit was identical with that of KT25 CTB, corresponding to the DNA sequence of ctxB from El Tor strains of the seventh pandemic. We concluded 1841 toxin was identical to CT of the seventh pandemic El Tor vibrios.
从孟加拉国一名腹泻患者分离出的霍乱弧菌O139菌株AI-1841中纯化出一种霍乱样肠毒素。将其特性与经典菌株569B和埃尔托菌株KT25的霍乱毒素（CTs）进行了比较。AI-1841产生的毒素与O1菌株一样多。这些毒素在常规聚丙烯酰胺凝胶圆盘电泳、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和等电聚焦中的迁移图谱以及它们对羟基磷灰石的亲和力方面没有区别。AI-1841毒素在兔回肠袢中诱导的皮肤渗透因子活性和液体蓄积与CTs相同。在蛋白质印迹法中，三种毒素与抗569B CT抗血清的反应相同，并且它们的B亚基通过双向凝胶免疫扩散与任何抗B亚基抗血清形成沉淀线。抗569B CTB抗体在其PF活性和肠毒性方面中和了这三种毒素。1841毒素B亚基的氨基酸序列与KT25 CTB的相同，对应于第七次大流行埃尔托菌株的ctxB的DNA序列。我们得出结论，1841毒素与第七次大流行埃尔托弧菌的CT相同。
