Rasheed B K, McLendon R E, Friedman H S, Friedman A H, Fuchs H E, Bigner D D, Bigner S H
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Oncogene. 1995 Jun 1;10(11):2243-6.
The high incidence of loss of chromosome 10 alleles in glioblastoma multiforme suggests the presence on this chromosome of a tumor suppressor gene that is important in glioma tumorigenesis and progression. Our initial deletion mapping studies using restriction fragment length polymorphism markers indicated a common deletion region in 10q24-qter. In an attempt to localize the deleted region further, we screened a panel of 117 gliomas for loss of heterozygosity for chromosome 10 loci using 10 microsatellite markers. Seventeen tumors showed partial loss of a copy of chromosome 10 and were further analysed using 28 additional microsatellite markers. Of these, 10 had terminal deletion in the q arm, three had deletions in both p and q arms, two contained interstitial deletion in 10q and two carried deletions in 10p. In the 15 tumors with deletions in 10q, the minimal overlapping deletion region was in distal 10q between markers D10S587 and D10S216. Loci D10S587 and D10S216 are approximately mapped to a 5 cM region in 10q25.1.
多形性胶质母细胞瘤中10号染色体等位基因缺失的高发生率表明，该染色体上存在一个在胶质瘤发生和进展中起重要作用的肿瘤抑制基因。我们最初使用限制性片段长度多态性标记进行的缺失定位研究表明，10q24 - qter存在一个常见的缺失区域。为了进一步定位缺失区域，我们使用10个微卫星标记筛选了一组117例胶质瘤，以检测10号染色体位点的杂合性缺失。17个肿瘤显示10号染色体的一个拷贝部分缺失，并使用另外28个微卫星标记进行了进一步分析。其中，10个在q臂有末端缺失，3个在p臂和q臂均有缺失，2个在10q有中间缺失，2个在10p有缺失。在15个10q有缺失的肿瘤中，最小重叠缺失区域位于标记D10S587和D10S216之间的10q远端。基因座D10S587和D10S216大致定位在10q25.1的一个5 cM区域。
