Evans A A, Hughes S, Smith M E
Department of Physiology, Medical School, University of Birmingham, UK.
Peptides. 1995;16(2):361-4. doi: 10.1016/0196-9781(94)00185-5.
Autoradiography was used to study the opioid receptors in soleus and extensor digitorum longus (EDL) muscles from normal mice and mice with type II diabetes. Binding sites for [125I]beta-endorphin were present on the surface membranes in muscles from normal mice. The density of receptors was higher in muscles from obese diabetic mice. The specific delta-opioid ligands DPDPE and [D-Ala2]deltorphin-II inhibited the binding of [125I]beta-endorphin whereas mu and kappa agonists did not. Therefore, the opioid receptor present in skeletal muscle fibers of the mouse is of the delta subtype and the number of these receptors is increased in type II diabetes in the mouse.
利用放射自显影术研究正常小鼠和II型糖尿病小鼠比目鱼肌及趾长伸肌（EDL）中的阿片受体。正常小鼠肌肉的表面膜上存在[125I]β-内啡肽的结合位点。肥胖糖尿病小鼠肌肉中的受体密度更高。特异性δ-阿片配体DPDPE和[D-Ala2]δ-内啡肽-II可抑制[125I]β-内啡肽的结合，而μ和κ激动剂则不能。因此，小鼠骨骼肌纤维中存在的阿片受体为δ亚型，且在小鼠II型糖尿病中这些受体的数量增加。
