Chamson A, Bertheas M F, Frey J
Laboratoire de Biochimie, Faculté de Médecine, Saint Etienne, France.
Prenat Diagn. 1995 Feb;15(2):165-70. doi: 10.1002/pd.1970150210.
After chorionic villus biopsy of human placenta, cell cultures were propagated with Ham's F10 medium or Eagle's minimum essential medium (MEM). It was possible to study the morphology of the cells by transmission electron microscopy (TEM) after a special culture of the cells in a collagen gel. The cells embedded in a collagen gel were able to contract the gel and to organize collagen fibres, as fibroblast cultures do. TEM showed vacuolization and well-developed cisternae of the endoplasmic reticulum, especially in the case of MEM culture. The aim was to determine whether cells cultivated from early placenta were able to synthesize enough collagen for a metabolic study. A high level of collagen biosynthesis could be quantified. Types I and III collagen can be determined which is useful for studying the abnormalities of collagen synthesis in suspected cases of osteogenesis imperfecta or Ehlers-Danlos type IV syndrome. The hydroxylation of lysine can also be studied with respect to Ehlers-Danlos type VI syndrome. Moreover, these cells, in contrast to fibroblast cultures, made it possible to study the biosynthesis of type IV collagen.
对人胎盘进行绒毛取样后，细胞培养物在哈姆氏F10培养基或伊格尔氏基本培养基（MEM）中增殖。在胶原凝胶中对细胞进行特殊培养后，通过透射电子显微镜（TEM）研究细胞形态成为可能。嵌入胶原凝胶中的细胞能够像成纤维细胞培养物那样收缩凝胶并组织胶原纤维。透射电子显微镜显示有液泡化现象以及内质网池发育良好，尤其是在MEM培养的情况下。目的是确定从早期胎盘培养的细胞是否能够合成足够的胶原蛋白用于代谢研究。可以对高水平的胶原蛋白生物合成进行定量。可以确定I型和III型胶原蛋白，这对于研究疑似成骨不全症或IV型埃勒斯-当洛综合征病例中的胶原蛋白合成异常很有用。关于VI型埃勒斯-当洛综合征，还可以研究赖氨酸的羟化作用。此外，与成纤维细胞培养物不同，这些细胞使得研究IV型胶原蛋白的生物合成成为可能。
