Nissinen A, Seppälä H, Huovinen P
Antimicrobial Research Laboratory, National Public Health Institute, Turku, Finland.
Scand J Infect Dis. 1995;27(1):52-6. doi: 10.3109/00365549509018972.
Erythromycin susceptibility of clinical Streptococcus pyogenes isolates was determined at 19 Finnish clinical microbiology laboratories by their routine disk diffusion method and by a screening method adapted from the breakpoint susceptibility testing method. Results obtained at 12 laboratories using 4 major variants of the disk method were further evaluated. From these laboratories, 286 consecutive resistant and 349 consecutive susceptible isolates were sent to the Antimicrobial Research Laboratory, Turku where the MIC of erythromycin was determined. 96% and 97% of the disk results were correct, as compared with MIC results, when general and laboratory-specific breakpoints, respectively, were used. The results of the screening method were comparable to those of the disk method.
芬兰19家临床微生物实验室采用常规纸片扩散法及一种改编自折点药敏试验方法的筛查方法，对临床分离的化脓性链球菌进行红霉素药敏试验。对12家采用4种主要纸片法变体的实验室所获结果作进一步评估。从这些实验室选取286株连续的耐药菌株及349株连续的敏感菌株送至图尔库抗菌研究实验室，测定红霉素的最低抑菌浓度（MIC）。当分别采用通用折点和实验室特定折点时，与MIC结果相比，纸片法结果的正确率分别为96%和97%。筛查方法的结果与纸片法结果相当。
